MicroRNA-379-5p regulates free cholesterol accumulation and relieves diet induced-liver damage in db/db mice via STAT1/HMGCS1 axis
文献类型：期刊论文
| 作者 | Dong, Yunxia1,2; Yu, Chuwei1,2; Ma, Ningning1,2,3; Xu, Xiaoding1,2,4; Wu, Qian1; Lu, Henglei1; Gong, Likun1,2 ; Chen, Jing1,2; Ren, Jin1,2,4
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| 刊名 | Molecular biomedicine
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| 出版日期 | 2022-08-10 |
| 卷号 | 3期号:1页码:25 |
| 关键词 | Cholesterol metabolism HMGCS1 MiR-379-5p Non-alcoholic fatty liver disease STAT1 |
| ISSN号 | 2662-8651 |
| DOI | 10.1186/s43556-022-00089-w |
| 文献子类 | Article |
| 英文摘要 | Lipotoxicity induced by the overload of lipid in the liver, especially excess free cholesterol (FC), has been recognized as one of driving factors in the transition from non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH). MicroRNA (miR)-379-5p has been reported to play regulatory roles in hepatic triglyceride homeostasis, but the relationship of miR-379-5p and hepatic cholesterol homeostasis has never been touched. In the current study, we found that hepatic miR-379-5p levels were decreased obviously in NAFLD patients and model mice compared with their controls. Moreover, miR-379-5p was discovered to be able to inhibit intracellular FC accumulation and alleviate mitochondrial damage induced by palmitic acid (PA) in vitro. Furthermore, overexpression of miR-379-5p in HFHC-fed db/db mice could reduce the level of hepatic total cholesterol (TC) and FC, and ameliorate hepatic injury reflected by the lower serum alanine aminotransferase (ALT) and aspartate transaminase (AST). Subsequently, by combining spectrometry (MS) and luciferase assay, we identified miR-379-5p suppressed STAT1 through transcriptional and translational regulation. Finally, we confirmed that STAT1 was a transcriptional factor of HMGCS1. In conclusion, miR-379-5p inhibits STAT1 expression and regulates cholesterol metabolism through the STAT1/HMGCS1 axis, suggesting miR-379-5p might be applied to improve lipotoxicity in the future. |
| 语种 | 英语 |
| WOS记录号 | MEDLINE:35945406 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309288]  |
| 专题 | 新药研究国家重点实验室 |
| 作者单位 | 1.Center for Drug Safety Evaluation and Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai, 201203, China; 2.University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, China; 3.School of Life Science and Technology, ShanghaiTech University, 100 Haike Road, Shanghai, 201210, China 4.School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, 210023, China |
| 推荐引用方式 GB/T 7714 | Dong, Yunxia,Yu, Chuwei,Ma, Ningning,et al. MicroRNA-379-5p regulates free cholesterol accumulation and relieves diet induced-liver damage in db/db mice via STAT1/HMGCS1 axis[J]. Molecular biomedicine,2022,3(1):25. |
| APA | Dong, Yunxia.,Yu, Chuwei.,Ma, Ningning.,Xu, Xiaoding.,Wu, Qian.,...&Ren, Jin.(2022).MicroRNA-379-5p regulates free cholesterol accumulation and relieves diet induced-liver damage in db/db mice via STAT1/HMGCS1 axis.Molecular biomedicine,3(1),25. |
| MLA | Dong, Yunxia,et al."MicroRNA-379-5p regulates free cholesterol accumulation and relieves diet induced-liver damage in db/db mice via STAT1/HMGCS1 axis".Molecular biomedicine 3.1(2022):25. |
入库方式： OAI收割
来源：上海药物研究所
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