Dehydroberberine Analogue Nanoassemblies for Inducing and Self-Reporting Mitochondrial Dysfunction in Tumor Cells
文献类型：期刊论文
| 作者 | Zhang, Rui1,2; An, Ruibing3; Gu, Zhanni1; Sun, Haifeng1; Ye, Deju3; Liu, Hong1,2
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| 刊名 | ACS applied bio materials
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| 出版日期 | 2021-03-15 |
| 卷号 | 4期号:3页码:2033-2043 |
| 关键词 | dehydroberberine fluorescence mitochondria self-assembly self-reporting |
| ISSN号 | 2576-6422 |
| DOI | 10.1021/acsabm.0c00747 |
| 文献子类 | Article |
| 英文摘要 | Mitochondria-targeting probes that allow us to induce and report mitochondrial dysfunction have become promising theranostic agents for cancer; however, the lack of selectivity toward tumor cells over normal tissue cells has impeded the treatment outcome. Herein, we develop 10 fluorescent dehydroberberine derivatives (B1-B10) capable of lighting up mitochondria and exerting moderate cytotoxicity against tumor cells. To enable the selectivity toward tumor cells over normal tissue cells, we introduced a lipophilic anion tetraphenylborate (TPB-) into the most potent compound B3+Cl- to drive molecular self-assembly into monodisperse organic nanoassemblies (B3NPs) in aqueous solution, which efficiently enhance the delivery of B3+ into HeLa cells assisted by an electrostatic interaction-driven anion-exchange process. Fluorescence imaging reveals that B3+ can initially accumulate in the mitochondria after entering HeLa cells, followed by inducing mitochondrial dysfunction and then migrating into the nucleus. Strong B3+ fluorescence translocating from mitochondria to nucleus can be monitored in real-time, allowing for self-reporting of mitochondrial dysfunction in HeLa cells. Moreover, we demonstrate that B3NPs exert significantly higher cytotoxicity against seven different tumor cells (e.g., U87MG, HeLa, MDA-MB-468, MDA-MB-435, MDA-MB-231, MCF-7, and HCT116 cells) compared to human normal tissue cells (e.g., HUVEC, HEK293). This work highlights the utility of the self-assembly approach to improve the cytotoxicity and selectivity of mitochondria-targeting agents against tumor cells. |
| 语种 | 英语 |
| WOS记录号 | MEDLINE:35014329 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309293]  |
| 专题 | 新药研究国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Drug Research and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China.; 2.University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China.; 3.State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (Chem-BIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. |
| 推荐引用方式 GB/T 7714 | Zhang, Rui,An, Ruibing,Gu, Zhanni,et al. Dehydroberberine Analogue Nanoassemblies for Inducing and Self-Reporting Mitochondrial Dysfunction in Tumor Cells[J]. ACS applied bio materials,2021,4(3):2033-2043. |
| APA | Zhang, Rui,An, Ruibing,Gu, Zhanni,Sun, Haifeng,Ye, Deju,&Liu, Hong.(2021).Dehydroberberine Analogue Nanoassemblies for Inducing and Self-Reporting Mitochondrial Dysfunction in Tumor Cells.ACS applied bio materials,4(3),2033-2043. |
| MLA | Zhang, Rui,et al."Dehydroberberine Analogue Nanoassemblies for Inducing and Self-Reporting Mitochondrial Dysfunction in Tumor Cells".ACS applied bio materials 4.3(2021):2033-2043. |
入库方式： OAI收割
来源：上海药物研究所
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