苦瓜 McOSC 和 McP450 的功能鉴定及在三萜 生物合成中的初步应用
文献类型：学位论文
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| 作者 | 韩雨桐 |
| 答辩日期 | 2021-05 |
| 导师 | 杨雪飞 |
| 英文摘要 | Triterpenoids as a research focus of natural products have anti-tumor, anti-inflammatory, anti-viral, anti-hypertensive, lipid-regulating, hypoglycemic, anti-AIDS and other activities. It was found that compared with monocotyledonous plants, triterpenoids were more widely distributed and varied in dicotyledonous plants, especially in Cucurbitaceae, Acanthaceae, Leguminosae and Aesculaceae. Momordica charantia L. is an annual climbing herb of Cucurbitaceae, also known as bitter gourd and bitter melon. As a common vegetable, its fruit contains multiple triterpenoids with functions of hypoglycemia, anti-obesity, anti-diabetes, anti-cancer and anti-gluconeogenesis. Due to the low efficiency of separation, extraction or chemical synthesis of triterpenoids in bitter gourd, their applications have been limited. Therefore, in recent years, studies on triterpenoids in bitter gourd have shifted to biosynthesis pathway. In this study, the transcriptome and triterpenoid metabolites at different growth stages of bitter gourd were analyzed; the expression patterns of triterpenoid synthesis candidate gene were explored. The McOSC and McP450 that formed gene clusters adjacent to each other were screened by chromosomal localization, and their functions in the synthesis of triterpenoids in bitter gourd were analyzed by exogenous gene expression test, which laid a foundation for the subsequent heterologous expression of triterpenoids in bitter gourd. The main results are as follows: （1）Transcriptome and metabolome of bitter gourd during development （2）Identification and expression pattern analysis of triterpenoid synthetic genes in bitter gourd （3）Function of McOSC and McP450 genes in bitter gourd |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74554]  |
| 专题 | 昆明植物研究所_昆明植物所硕博研究生毕业学位论文 |
| 推荐引用方式 GB/T 7714 | 韩雨桐. 苦瓜 McOSC 和 McP450 的功能鉴定及在三萜 生物合成中的初步应用, Functional identification of McOSC and McP450 from bitter gourd（Momordica charantia L.）and its preliminary application in triterpenoid biosynthesis[D]. 2021. |
入库方式： OAI收割
来源：昆明植物研究所
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