芜菁春化开花途径关键基因的功能分析
文献类型：学位论文
| ; | |
| 作者 | 高泽安 |
| 答辩日期 | 2021-06 |
| 导师 | 杨永平 |
| 英文摘要 | Turnip (Brassica rapa var. rapa), also known as Manjing, is an annual herb of Cruciferae, belonging to cabbage vegetables. The edible part of turnip is fleshy tuber, and the flowering process has obvious vernalization response. In actual production, premature bolting and flowering of turnip seriously affects the growth and development of fleshy tuber, resulting in the decline of quality and yield. Therefore, the analysis of the molecular mechanism of the vernalization and flowering pathway of turnip will provide significance to ensure the agricultural production of turnip. Vernalization refers to the phenomenon that some plants have to go through a period of continuous low temperature to change from vegetative growth to reproductive growth. In Arabidopsis thaliana, the key genes of vernalization and flowering pathway are FRI, FLC, FT and SOC1. Although Brassica rapa belongs to Cruciferae, the whole genome triploid event, which occurred in the evolutionary process, resulted in multiple copies of genes. In this study, through analysis of vernalization response phenotype of turnip, cloning the homologous genes BrrFRI, BrrFLC, BrrFT and BrrSOC1, constrcting the phylogenetic tree, analysis of the temporal and spatial expression of homologous genes, and genetic verification of gene function, we determine the main regulatory genes of vernalization flowering pathway in turnip and analyze their molecular mechanism. The results show that: (1) Tibetan turnip B17 needs vernalization for more than 30 days to induce bolting and flowering. When the vernalization treatment was less than 30 days, vegetative growth will be maintained and reproductive transformation will not be possible. With the extension of vernalization time, the time needing for bolting was gradually shortened, and the vegetative reproduction was inhibited. There were two copies of FRI gene, four copies of FLC gene, four copies of FT gene and four copies of SOC1 gene. Phylogenetic tree was constructed with homologues of Arabidopsis thaliana and Chinese cabbage (B. rapa spp pekinensis). It was found that the turnip 芜菁春化开花途径关键基因的功能分析 IV homologous genes were very close to those of Chinese cabbage. (2) Through analysis of spatiotemporal expression, vernalization response and subcellular localization of BrrFLC, BrrFRI, BrrSOC1 and BrrFT homologues of turnip, it was found that all of four BrrFLC homologues were highly expressed in the leaves; BrrFRIa and BrrFRIb were mainly expressed in leaves and flowered leaves; BrrSOC1 and BrrFT homologues were highly expressed in leaves and flowered leaves. During different growth stages, the expression levels of BrrFLC, BrrFRI, BrrSOC1 and BrrFT homologues first increased and then decreased, but the stage of peak expression was different. In the process of vernalization, the expression of BrrFLC genes were inhibited by vernalization.Both BrrSOC1 and BrrFT homologous genes were activated after vernalization, especia |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74599]  |
| 专题 | 昆明植物研究所_昆明植物所硕博研究生毕业学位论文 |
| 推荐引用方式 GB/T 7714 | 高泽安. 芜菁春化开花途径关键基因的功能分析, Functional analysis of key genes involved in vernalization and flowering pathway in Turnip (Brassica rapa var. rapa）[D]. 2021. |
入库方式： OAI收割
来源：昆明植物研究所
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