Integrative Proteomic Analysis Reveals the Cytoskeleton Regulation and Mitophagy Difference Between Ischemic Cardiomyopathy and Dilated Cardiomyopathy
文献类型：期刊论文
| 作者 | Liu, Muyin1,2,3,4; Zhai, Linhui4,5,6; Yang, Zhaohua7; Li, Su1,2,3; Liu, Tianxian4; Chen, Ao1,2,3; Wang, Lulu4; Li, Youran1,2,3; Li, Ruidong; Li, Chenguang1,2,3 |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
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| 出版日期 | 2023-12-01 |
| 卷号 | 22期号:12页码:19 |
| DOI | 10.1016/j.mcpro.2023.100667 |
| 通讯作者 | Chen, Zhangwei(chen.zhangwei@zs-hospital.sh.cn) ; Qian, Juying(qian.juying@zs-hospital.sh.cn) |
| 英文摘要 | Ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM) are the two primary etiologies of end -stage heart failure. However, there remains a dearth of comprehensive understanding the global perspective and the dynamics of the proteome and phosphoproteome in ICM and DCM, which hinders the profound comprehension of pivotal biological characteristics as well as differences in signal transduction activation mechanisms between these two major types of heart failure. We conducted highthroughput quantification proteomics and phosphoproteomics analysis of clinical heart tissues with ICM or DCM, which provided us the system -wide molecular insights into pathogenesis of clinical heart failure in both ICM and DCM. Both protein and phosphorylation expression levels exhibit distinct separation between heart failure and normal control heart tissues, highlighting the prominent characteristics of ICM and DCM. By integrating with omics results, Western blots, phosphosite-specific mutation, chemical intervention, and immunofluorescence validation, we found a significant activation of the PRKACA-GSK3 beta signaling pathway in ICM. This signaling pathway influenced remolding of the microtubule network and regulated the critical actin filaments in cardiac construction. Additionally, DCM exhibited significantly elevated mitochondria energy supply injury compared to ICM, which induced the ROCK1-vimentin signaling pathway activation and promoted mitophagy. Our study not only delineated the major distinguishing features between ICM and DCM but also revealed the crucial discrepancy in the mechanisms between ICM and DCM. This study facilitates a more profound comprehension of pathophysiologic heterogeneity between ICM and DCM and provides a novel perspective to assist in the discovery of potential therapeutic targets for different types of heart failure. |
| WOS关键词 | MYOCARDIAL-INFARCTION ; ESC GUIDELINES ; MITOCHONDRIAL ; DIAGNOSIS ; AUTOPHAGY |
| 资助项目 | National Key R&D Program of China[2020YFE0202200] ; National Natural Science Foundation of China[22225702] ; National Natural Science Foundation of China[32071432] ; National Natural Science Foundation of China[32171434] ; Program of Shanghai Academic Research Leader[22XD1420900] ; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, China[KF-202201] ; Innovative Research Team of High -Level Local Universities in Shanghai[SHSMU-ZDCX20212700] ; Shanghai Municipal Key Clinicial Specialty[shslczdzk06201] ; Shanghai Clinical Research Center for Interventional Medicine[19MC1910300] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001162966000001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309569]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Zhangwei; Qian, Juying |
| 作者单位 | 1.Fudan Univ, Zhongshan Hosp, Shanghai Inst Cardiovasc Dis, Dept Cardiol, Shanghai, Peoples R China 2.Shanghai Inst Cardiovasc Dis, Shanghai, Peoples R China 3.Natl Clin Res Ctr Intervent Med, Shanghai, Peoples R China 4.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai, Peoples R China 5.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan, Peoples R China 6.Tongji Univ, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Like Intelligence, Sch Med, Shanghai, Peoples R China 7.Jiangsu Ocean Univ, Sch Pharm, Lianyungang, Peoples R China 8.Jiangsu Ocean Univ, Coll Pharm, Lianyungang, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Muyin,Zhai, Linhui,Yang, Zhaohua,et al. Integrative Proteomic Analysis Reveals the Cytoskeleton Regulation and Mitophagy Difference Between Ischemic Cardiomyopathy and Dilated Cardiomyopathy[J]. MOLECULAR & CELLULAR PROTEOMICS,2023,22(12):19. |
| APA | Liu, Muyin.,Zhai, Linhui.,Yang, Zhaohua.,Li, Su.,Liu, Tianxian.,...&Qian, Juying.(2023).Integrative Proteomic Analysis Reveals the Cytoskeleton Regulation and Mitophagy Difference Between Ischemic Cardiomyopathy and Dilated Cardiomyopathy.MOLECULAR & CELLULAR PROTEOMICS,22(12),19. |
| MLA | Liu, Muyin,et al."Integrative Proteomic Analysis Reveals the Cytoskeleton Regulation and Mitophagy Difference Between Ischemic Cardiomyopathy and Dilated Cardiomyopathy".MOLECULAR & CELLULAR PROTEOMICS 22.12(2023):19. |
入库方式： OAI收割
来源：上海药物研究所
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