Inner nuclear membrane protein TMEM201 maintains endothelial cell migration and angiogenesis by interacting with the LINC complex
文献类型：期刊论文
| 作者 | Zhang, Yutian1,2; Kong, Ya1,2; Guo, Haoran1,2; Liu, Yun3; Zang, Yi1,2,4 ; Li, Jia1,2,3,4,5
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| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2022-07-14 |
| 卷号 | 14期号:3页码:mjac017 |
| 关键词 | transmembrane protein 201 inner nuclear membrane LINC complex endothelial cell migration angiogenesis |
| ISSN号 | 1674-2788 |
| DOI | 10.1093/jmcb/mjac017 |
| 文献子类 | Article |
| 英文摘要 | The nuclear envelope comprises the outer nuclear membrane, inner nuclear membrane (INM), and nucleopore. Although similar to 60 INM proteins have been identified, only a few of them have been well characterized, revealing their crucial roles. Our group focused on the INM protein transmembrane protein 201 (TMEM201), whose role in cellular function remains to be defined. In this study, we investigated the role of TMEM201 in endothelial cell migration and angiogenesis. Depletion of TMEM201 expression by short hairpin RNA-mediated interference impeded human umbilical vein endothelial cell (HUVEC) angiogenic behavior in tube formation and fibrin gel bead sprouting assays. Meanwhile, TMEM201-deficient HUVECs exhibited impaired migration ability. We next explored the underlying mechanism and found that the N-terminal of TMEM201 interacted with the linker of nucleoskeleton and cytoskeleton complex and was required for regulating endothelial cell migration and angiogenesis. These in vitro findings were further confirmed by using in vivo models. In Tmem201-knockout mice, retinal vessel development was arrested and aortic ring sprouting was defective. In addition, loss of tmem201 impaired zebrafish intersegmental vessel development. In summary, TMEM201 was shown to regulate endothelial cell migration and control the process of angiogenesis. This study is the first to reveal the role of INM proteins in the vascular system and angiogenesis. |
| WOS关键词 | IN-VITRO ; ENVELOPE ; SAMP1 ; MOUSE ; EXPRESSION ; NESPRIN-1 ; LAMINS ; MODEL |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000825006000001 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309319]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zang, Yi; Li, Jia |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.China Pharmaceut Univ, State Key Lab Nat Med, Jiangsu Key Lab Drug Screening, Nanjing 210009, Peoples R China; 4.Univ Chinese Acad Sci, Sch Pharmaceut Sci & Technol, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China; 5.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Druggabil Res Marine Nat Prod, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yutian,Kong, Ya,Guo, Haoran,et al. Inner nuclear membrane protein TMEM201 maintains endothelial cell migration and angiogenesis by interacting with the LINC complex[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2022,14(3):mjac017. |
| APA | Zhang, Yutian,Kong, Ya,Guo, Haoran,Liu, Yun,Zang, Yi,&Li, Jia.(2022).Inner nuclear membrane protein TMEM201 maintains endothelial cell migration and angiogenesis by interacting with the LINC complex.JOURNAL OF MOLECULAR CELL BIOLOGY,14(3),mjac017. |
| MLA | Zhang, Yutian,et al."Inner nuclear membrane protein TMEM201 maintains endothelial cell migration and angiogenesis by interacting with the LINC complex".JOURNAL OF MOLECULAR CELL BIOLOGY 14.3(2022):mjac017. |
入库方式： OAI收割
来源：上海药物研究所
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