A Tandemly Activated Fluorescence Probe for Detecting Senescent Cells with Improved Selectivity by Targeting a Biomarker Combination
文献类型：期刊论文
| 作者 | Zhou, Lei1,2; Zhang, Xuan3,4 ; Dong, Ying3,4; Pan, Yuan1; Li, Jia3,4,5; Zang, Yi3,4,5; Li, Xin1
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| 刊名 | ACS SENSORS
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| 出版日期 | 2022-06 |
| 关键词 | fluorescent probes fluorescent imaging senescent cells formaldehyde figalactosidase selectivity |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.2c00719 |
| 文献子类 | Article |
| 英文摘要 | ABSTRACT: The heterogeneous and complex phenotypes of cellular senescence necessitate a biomarker combination for the accurate detection of senescent cells from others. However, this raises the challenge of detecting multiple senescent biomarkers in the same live cell simultaneously. Herein we reported the strategy of biomarker combination triggered tandem activation for designing senescencespecific fluorogenic probes, which resulted in the development of the probe PGal_FA. The fluorescence of PGal_FA can only be activated by the sequential stimulation by the senescent biomarker combination of figalactosidase (fiGal) and formaldehyde (FA), with fiGal activating the sensing ability of the probe toward FA. Facilitated by probe PGal_FA, the simultaneous detection of a biomarker combination in the same live cell was realized. We have demonstrated the improved selectivity of probe PGal_FA toward senescent cells compared to the traditional single-biomarker-based probe. Probe PGal_FA was also successfully used to detect senescent cells in bleomycin-induced pulmonary fibrosis tissues. We expect probe PGal_FA to be a reliable tool for the study on cellular senescence and envision that this probe design strategy may be expanded to other biological events to improve accuracy. |
| WOS关键词 | CELLULAR SENESCENCE ; SECRETORY PHENOTYPE ; IN-VIVO ; FORMALDEHYDE ; CLEARANCE ; CANCER |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000823478200001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309333]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zang, Yi; Li, Xin |
| 作者单位 | 1.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Peoples R China; 2.Zhejiang Univ Technol, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 5.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Lei,Zhang, Xuan,Dong, Ying,et al. A Tandemly Activated Fluorescence Probe for Detecting Senescent Cells with Improved Selectivity by Targeting a Biomarker Combination[J]. ACS SENSORS,2022. |
| APA | Zhou, Lei.,Zhang, Xuan.,Dong, Ying.,Pan, Yuan.,Li, Jia.,...&Li, Xin.(2022).A Tandemly Activated Fluorescence Probe for Detecting Senescent Cells with Improved Selectivity by Targeting a Biomarker Combination.ACS SENSORS. |
| MLA | Zhou, Lei,et al."A Tandemly Activated Fluorescence Probe for Detecting Senescent Cells with Improved Selectivity by Targeting a Biomarker Combination".ACS SENSORS (2022). |
入库方式： OAI收割
来源：上海药物研究所
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