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A long-wavelength fluorescent probe with a large Stokes shift for lysosome-targeted imaging of Cys and GSH

文献类型：期刊论文

作者Sun, Yi-Hang1,2; Han, Hai-Hao1,2,3; Huang, Jia-Min1,2; Li, Jia3; Zang, Yi3; Wang, Cheng-Yun1,2
刊名SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
出版日期2021-11-15
卷号261页码:120055
ISSN号1386-1425
关键词Fluorescent probe Lysosome targeting Cysteine Glutathione Near Infrared Emission Bioimaging
DOI10.1016/j.saa.2021.120055
文献子类Article
英文摘要Biothiols including cysteine (Cys) and glutathione (GSH) are biological signaling molecules responsible for cell detoxification, cell metabolism and neutralization of reactive oxygen species. Here, we synthe-sized a long-wavelength fluorescent probe, DCIMA, for lysosome-targeted imaging of Cys and GSH in liv-ing cells. DCIMA is consisted of a dicyanoisophorone core modified with an acrylate group for biothiol detection through the Michael addition reaction and a morpholine group as the lysosome-targeting agent. The presence of the electron-donating morpholine group also enhances the intramolecular charge transfer mechanism of the probe, thereby enabling its long-wavelength fluorescence emission (670 nm) and large Stokes shift (180 nm). In concentration range of 0-30 mu M, the probe was determined to react quickly with both Cys and GSH with low detection limits (<5 min, 35.2 nM for GSH and 34.8 nM for Cys) and achieve the sensitive fluorescence imaging of the biothiols located in the lysosomes of living cells. (C) 2021 Elsevier B.V. All rights reserved.
WOS关键词ON PROBE ; IN-VIVO ; CYSTEINE ; GLUTATHIONE ; CHEMOTHERAPY ; CELLS
WOS研究方向Spectroscopy
语种英语
出版者PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号WOS:000670305400010
源URL[http://119.78.100.183/handle/2S10ELR8/309382]  
专题新药研究国家重点实验室
通讯作者Zang, Yi; Wang, Cheng-Yun
作者单位1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;
2.East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China;
3.Chinese Acad Sci, Natl Ctr Drug Screening, Inst Mat Med, State Key Lab Drug Res, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Yi-Hang,Han, Hai-Hao,Huang, Jia-Min,et al. A long-wavelength fluorescent probe with a large Stokes shift for lysosome-targeted imaging of Cys and GSH[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2021,261:120055.
APA Sun, Yi-Hang,Han, Hai-Hao,Huang, Jia-Min,Li, Jia,Zang, Yi,&Wang, Cheng-Yun.(2021).A long-wavelength fluorescent probe with a large Stokes shift for lysosome-targeted imaging of Cys and GSH.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,261,120055.
MLA Sun, Yi-Hang,et al."A long-wavelength fluorescent probe with a large Stokes shift for lysosome-targeted imaging of Cys and GSH".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 261(2021):120055.

入库方式： OAI收割

来源：上海药物研究所

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