Single-cell lipidomics with high structural specificity by mass spectrometry
文献类型：期刊论文
| 作者 | Li, Zishuai1; Cheng, Simin1; Lin, Qiaohong2; Cao, Wenbo1; Yang, Jing1; Zhang, Minmin3 ; Shen, Aijun3; Zhang, Wenpeng1; Xia, Yu2; Ma, Xiaoxiao1
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| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2021-05-17 |
| 卷号 | 12期号:1页码:2869 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/s41467-021-23161-5 |
| 文献子类 | Article |
| 英文摘要 | Single-cell analysis is critical to revealing cell-to-cell heterogeneity that would otherwise be lost in ensemble analysis. Detailed lipidome characterization for single cells is still far from mature, especially when considering the highly complex structural diversity of lipids and the limited sample amounts available from a single cell. We report the development of a general strategy enabling single-cell lipidomic analysis with high structural specificity. Cell fixation is applied to retain lipids in the cell during batch treatments prior to single-cell analysis. In addition to tandem mass spectrometry analysis revealing the class and fatty acyl-chain for lipids, batch photochemical derivatization and single-cell droplet treatment are performed to identify the C=C locations and sn-positions of lipids, respectively. Electro-migration combined with droplet-assisted electrospray ionization enables single-cell mass spectrometry analysis with easy operation but high efficiency in sample usage. Four subtypes of human breast cancer cells are correctly classified through quantitative analysis of lipid C=C location or sn-position isomers in similar to 160 cells. Most importantly, the single-cell deep lipidomics strategy successfully discriminates gefitinib-resistant cells from a population of wild-type human lung cancer cells (HCC827), highlighting its unique capability to promote precision medicine. |
| WOS关键词 | UNSATURATED FATTY-ACIDS ; C=C LOCATION ISOMERS ; LUNG-CANCER ; IDENTIFICATION ; QUANTITATION ; CLASSIFICATION ; PHOSPHOLIPIDS ; METABOLOMICS ; EPOXIDATION ; RESISTANCE |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATURE RESEARCH |
| WOS记录号 | WOS:000658736000012 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309388]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Ma, Xiaoxiao; Ouyang, Zheng |
| 作者单位 | 1.Tsinghua Univ, Dept Precis Instrument, State Key Lab Precis Measurement Technol & Instru, Beijing, Peoples R China; 2.Tsinghua Univ, Dept Chem, MOE Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai, Peoples R China; 4.Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA |
| 推荐引用方式 GB/T 7714 | Li, Zishuai,Cheng, Simin,Lin, Qiaohong,et al. Single-cell lipidomics with high structural specificity by mass spectrometry[J]. NATURE COMMUNICATIONS,2021,12(1):2869. |
| APA | Li, Zishuai.,Cheng, Simin.,Lin, Qiaohong.,Cao, Wenbo.,Yang, Jing.,...&Ouyang, Zheng.(2021).Single-cell lipidomics with high structural specificity by mass spectrometry.NATURE COMMUNICATIONS,12(1),2869. |
| MLA | Li, Zishuai,et al."Single-cell lipidomics with high structural specificity by mass spectrometry".NATURE COMMUNICATIONS 12.1(2021):2869. |
入库方式： OAI收割
来源：上海药物研究所
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