Aristolochic acid I induces impairment in spermatogonial stem cell in rodents
文献类型：期刊论文
| 作者 | Liu, Yongzhen1,2 ; He, Xiang3; Wang, Yuli2; Zhou, Houzu2; Zhang, Yuan2; Ma, Jianyun2; Wang, Zhaochu2; Yang, Fangfang2; Lu, Henglei1,2; Yang, Yifu3
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| 刊名 | TOXICOLOGY RESEARCH
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| 出版日期 | 2021-04-26 |
| 卷号 | 10期号:3页码:436-445 |
| 关键词 | aristolochic acid I spermatogenesis spermatogonial stem cells apoptosis P53 BAX |
| ISSN号 | 2045-452X |
| DOI | 10.1093/toxres/tfab038 |
| 文献子类 | Article |
| 英文摘要 | Aristolochic acid I (AAI) is a natural bioactive substance found in plants from the Aristolochiaceae family and impairs spermatogenesis. However, whether AAI-induced spermatogenesis impairment starts at the early stages of spermatogenesis has not yet been determined. Spermatogonial stem cells (SSCs) are undifferentiated spermatogonia that balance self-renewing and differentiating divisions to maintain spermatogenesis throughout adult life and are the only adult stem cells capable of passing genes onto the next generation. The objective of this study was to investigate whether AAI impairs SSCs during the early stages of spermatogenesis. After AAI treatment, we observed looser, smaller and fewer colonies, decreased cell viability, a decreased relative cell proliferation index, and increased apoptosis in SSCs in a concentrationand/or time-dependent manner. Additionally, AAI promoted apoptosis in SSCs, which was accompanied by upregulation of caspase 3, P53 and BAX expression and downregulation of Bcl-2 expression, and suppressed autophagy, which was accompanied by upregulation of P62 expression and downregulation of ATGS and LC3B expression, in a concentration-dependent manner. Then we found that AAI impaired spermatogenesis in rats, as identified by degeneration of the seminiferous epithelium, and increased apoptosis of testicular cells. Taken together, our findings demonstrate that AAI causes damage to SSCs and implicate apoptosis and autophagy in this process. The impairment of SSCs may contribute to AAI-induced testicular impairment. Our findings provide crucial information for the human application of botanical products containing trace amounts of AAI. |
| WOS关键词 | TUMOR-SUPPRESSOR P53 ; SERTOLI-CELLS ; DNA-DAMAGE ; BAX ; APOPTOSIS ; MOUSE ; ACTIVATION ; GENE ; SPERMATOGENESIS ; TRANSCRIPTION |
| WOS研究方向 | Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000671151100008 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309391]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Deng, Zhongping; Qi, Xinming; Gong, Likun; Ren, Jin |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Ctr Drug Safety Evaluat & Res, Innovat Res Inst Tradit Chinese Med, 1200 Cailun Rd,Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, State Key Lab Drug Res, 501 Haike Rd,Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China; 3.Shanghai Univ Tradit Chinese Med, Expt Ctr Sci & Technol, Lab Immunol & Virol, 1200 Cailun Rd,Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Inst Drug Discovery & Dev, Bldg A, Zhongshan Tsuihang New D 528400, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yongzhen,He, Xiang,Wang, Yuli,et al. Aristolochic acid I induces impairment in spermatogonial stem cell in rodents[J]. TOXICOLOGY RESEARCH,2021,10(3):436-445. |
| APA | Liu, Yongzhen.,He, Xiang.,Wang, Yuli.,Zhou, Houzu.,Zhang, Yuan.,...&Ren, Jin.(2021).Aristolochic acid I induces impairment in spermatogonial stem cell in rodents.TOXICOLOGY RESEARCH,10(3),436-445. |
| MLA | Liu, Yongzhen,et al."Aristolochic acid I induces impairment in spermatogonial stem cell in rodents".TOXICOLOGY RESEARCH 10.3(2021):436-445. |
入库方式： OAI收割
来源：上海药物研究所
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