MiR-566 protects the malignant progression of breast cancer by negatively regulating WNT6
文献类型：期刊论文
| 作者 | Zhao, M-C1; Zhang, M-M2; Li, T.2; Tao, Z-H1; Du, Y-Q1; Wang, L-P1; Zhang, J.1; Wang, B-Y1; Hu, X-C1 |
| 刊名 | EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
 |
| 出版日期 | 2020-06 |
| 卷号 | 24期号:11页码:6185-6194 |
| 关键词 | MiR-566 WNT6 Breast cancer |
| ISSN号 | 1128-3602 |
| 文献子类 | Article |
| 英文摘要 | OBJECTIVE: To elucidate the relationship between microRNA-566 (miR-566) and prognosis in breast cancer (BC) and to clarify the influences of miR-566 and WNT6 in its locus region on BC progression. PATIENTS AND METHODS: MiR-566 and WNT6 levels in 44 pairs of BC samples were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The influences of miR-566 on clinical features and prognosis in BC patients were analyzed. According to the differential expressions of miR-566 in the tested BC cell lines, MDA-MB-231 and MCF-7 cells were selected for generating miR-566 knockdown and over-expression models, respectively. Cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU) and transwell assays were conducted to explore the role of miR-566 in BC cell functions. Besides, the regulatory interaction between miR-566 and its downstream gene WNT6 was assessed by performing Dual-Luciferase reporter assay. Finally, the co-regulation of miR-566 and WNT6 in BC cell functions was examined. RESULTS: MiR-566 was downregulated in BC tissues. BC patients with a low expression level of miR-566 were prone to suffering a large tumor size, advanced tumor grade, high incidence of lymphatic metastasis and poor prognosis. Overexpression of miR-566 weakened proliferative and migratory abilities in MCF-7 cells, whereas knockdown of miR-566 produced the opposite results in MDA-MB-231 cells. WNT6 was the target gene binding to miR-566. and they displayed a negative expression correlation in BC tissues. Regulatory effects of miR-566 on BC progression could be reversed by WNT6. CONCLUSIONS: MiR-566 is closely related to tumor size, tumor grade, lymphatic metastasis and prognosis in BC. It protects the malignant progression of BC by negatively regulating WNT6. |
| WOS关键词 | EPIDEMIOLOGY ; BIOMARKER ; MICRORNA |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000542190000039 |
| 出版者 | VERDUCI PUBLISHER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309414]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Hu, X-C |
| 作者单位 | 1.Fudan Univ, Shanghai Canc Ctr, Sch Med, Dept Med Oncol, Shanghai, Peoples R China; 2.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, M-C,Zhang, M-M,Li, T.,et al. MiR-566 protects the malignant progression of breast cancer by negatively regulating WNT6[J]. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,2020,24(11):6185-6194. |
| APA | Zhao, M-C.,Zhang, M-M.,Li, T..,Tao, Z-H.,Du, Y-Q.,...&Hu, X-C.(2020).MiR-566 protects the malignant progression of breast cancer by negatively regulating WNT6.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,24(11),6185-6194. |
| MLA | Zhao, M-C,et al."MiR-566 protects the malignant progression of breast cancer by negatively regulating WNT6".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 24.11(2020):6185-6194. |
入库方式： OAI收割
来源：上海药物研究所
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