miR-331-3p Suppresses Cell Proliferation in TNBC Cells by Downregulating NRP2
文献类型：期刊论文
| 作者 | Zhao, Mingchuan1; Zhang, Mengmeng2; Tao, Zhonghua1; Cao, Jun1; Wang, Leiping1; Hu, Xichun1 |
| 刊名 | TECHNOLOGY IN CANCER RESEARCH & TREATMENT
 |
| 出版日期 | 2020-03-13 |
| 卷号 | 19页码:2147483647 |
| 关键词 | miR-331-3p TNBC NRP2 |
| ISSN号 | 1533-0346 |
| DOI | 10.1177/1533033820905824 |
| 文献子类 | Article |
| 英文摘要 | Purpose: Triple-negative breast cancer is characterized by fast progression with high possible for metastasis and poor survival. Dysfunction of microRNAs plays an important role in the initiation and progression of cancer. Our previous microRNA-seq data indicated the downregulation of miR-331-3p in triple-negative breast cancer tissues compared with that of the noncancer tissues. However, the function of miR-331-3p in triple-negative breast cancer remains largely unknown. Herein, the involvement of miR-331-3p in triple-negative breast cancer was investigated and the therapeutic potential of miR-331-3p was also explored. Methods: Real-time quantitative polymerase chain reaction was performed to detect the expression of miR-331-3p in triple-negative breast cancer tissues and cell lines. The cell proliferation was determined by the cell counting kit-8 assay. Apoptosis of triple-negative breast cancer cells was examined by annexin V/propidium iodide staining. miRDB database was used to predict the potential targets of miR-331-3p. Western blot was performed to examine the expression of the target protein. Results: miR-331-3p was significantly downregulated in triple-negative breast cancer tissues and cell line. Lower miR-331-3p expression was significantly correlated with the tumor size, TNM stage, and lymph node metastasis of patients with triple-negative breast cancer. Functional experiments showed that the overexpression of miR-331-3p inhibited the proliferation and increased apoptosis of triple-negative breast cancer cells. Neuropilin-2 was identified as a target of miR-331-3p, which harbored binding site of miR-331-3p in its 3 '-untranslated region. Overexpression of miR-331-3p decreased the messenger RNA and protein levels of neuropilin-2 in triple-negative breast cancer cells. Restoration of neuropilin-2 partially reversed the inhibitory effects of miR-331-3p on the proliferation of triple-negative breast cancer cells. Conclusions: Our results demonstrated the novel function of miR-331-3p/neuropilin-2 signaling in regulating the malignant behaviors of triple-negative breast cancer cells, which suggested miR-331-3p as a potential target for the treatment of triple-negative breast cancer. |
| WOS关键词 | NEUROPILIN-2 ; CANCER ; EXPRESSION ; MICRORNA-331-3P ; THERAPIES ; CARCINOMA ; PATHWAYS ; TARGETS ; GROWTH ; HER2 |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000524260500001 |
| 出版者 | SAGE PUBLICATIONS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309417]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Hu, Xichun |
| 作者单位 | 1.Fudan Univ, Dept Med Oncol, Shanghai Canc Ctr, Sch Med, 270 Dongan Rd, Shanghai 200032, Peoples R China; 2.Chinese Acad Sci, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Mingchuan,Zhang, Mengmeng,Tao, Zhonghua,et al. miR-331-3p Suppresses Cell Proliferation in TNBC Cells by Downregulating NRP2[J]. TECHNOLOGY IN CANCER RESEARCH & TREATMENT,2020,19:2147483647. |
| APA | Zhao, Mingchuan,Zhang, Mengmeng,Tao, Zhonghua,Cao, Jun,Wang, Leiping,&Hu, Xichun.(2020).miR-331-3p Suppresses Cell Proliferation in TNBC Cells by Downregulating NRP2.TECHNOLOGY IN CANCER RESEARCH & TREATMENT,19,2147483647. |
| MLA | Zhao, Mingchuan,et al."miR-331-3p Suppresses Cell Proliferation in TNBC Cells by Downregulating NRP2".TECHNOLOGY IN CANCER RESEARCH & TREATMENT 19(2020):2147483647. |
入库方式： OAI收割
来源：上海药物研究所
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