Identification of chemotypes in bitter melon by metabolomics: a plant with potential benefit for management of diabetes in traditional Chinese medicine
文献类型：期刊论文
| 作者 | Zhou, Shuaizhen1,3,4; Allard, Pierre-Marie1; Wolfrum, Christian2; Ke, Changqiang3,4 ; Tang, Chunping3,4; Ye, Yang3,4; Wolfender, Jean-Luc1
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| 刊名 | METABOLOMICS
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| 出版日期 | 2019-07-18 |
| 卷号 | 15期号:8页码:104 |
| 关键词 | Bitter melon Momordica charantia Molecular networking PCA UHPLC-HRMS Triterpenoids Metabolomics |
| ISSN号 | 1573-3882 |
| DOI | 10.1007/s11306-019-1565-7 |
| 文献子类 | Article |
| 英文摘要 | Introduction Bitter melon (Momordica charantia, Cucurbitaceae) is a popular edible medicinal plant, which has been used as a botanical dietary supplement for the treatment of diabetes and obesity in Chinese folk medicine. Previously, our team has proved that cucurbitanes triterpenoid were involved in bitter melon's anti-diabetic effects as well as on increasing energy expenditure. The triterpenoids composition can however be influenced by changes of varieties or habitats. Objectives To clarify the significance of bioactive metabolites diversity among different bitter melons and to provide a guideline for selection of bitter melon varieties, an exploratory study was carried out using a UHPLC-HRMS based metabolomic study to identify chemotypes. Methods Metabolites of 55 seed samples of bitter melon collected in different parts of China were profiled by UHPLC-HRMS. The profiling data were analysed with multivariate (MVA) statistical methods. Principle component analysis (PCA) and hierarchical cluster analysis (HCA) were applied for sample differentiation. Marker compounds were identified by comparing spectroscopic data with isolated compounds, and additional triterpenes were putatively identified by propagating annotations through a molecular network (MN) generated from UHPLC-HRMS & MS/MS metabolite profiling. Results PCA and HCA provided a good discrimination between bitter melon samples from various origins in China. This study revealed for the first time the existence of two chemotypes of bitter melon. Marker compounds of those two chemotypes were identified at different MSI levels. The combined results of MN and MVA demonstrated that the two chemotypes mainly differ in their richness in cucurbitane versus oleanane triterpenoid glycosides (CTGs vs. OTGs). Conclusion Our finding revealed a clear chemotype distribution of bioactive components across bitter melon varieties. While bioactivities of individual CTGs and OTGs still need to be investigated in more depth, our results could help in future the selection of bitter melon varieties with optimised metabolites profile for an improved management of diabetes with this popular edible Chinese folk medicine. |
| WOS关键词 | WHOLE FRUIT EXTRACT ; NATURAL-PRODUCTS ; FATTY LIVER ; CHARANTIA ; GLYCOSIDES ; SEED ; CONSTITUENTS ; SAPONINS ; PEEL ; PULP |
| WOS研究方向 | Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000476513800001 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309425]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Ye, Yang; Wolfender, Jean-Luc |
| 作者单位 | 1.Univ Geneva, Univ Lausanne, EPGL, Sch Pharmaceut Sci, CMU Rue Michel Servet 1, CH-1206 Geneva 4, Switzerland; 2.Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Swiss Fed Inst Technol, Schorenstr 16, CH-8603 Schwerzenbach, Switzerland; 3.Chinese Acad Sci, State Key Lab Drug Res, Nat Prod Chem Dept, Shanghai Inst Mat Med, 555 Zu Chong Zhi Rd,Zhangjiang High Tech Pk, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, SIMM, Nat Prod Chem Dept, CUHK Joint Res Lab Promoting Tradit Chinese Med, 555 Zu Chong Zhi Rd,Zhangjiang High Tech Pk, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Shuaizhen,Allard, Pierre-Marie,Wolfrum, Christian,et al. Identification of chemotypes in bitter melon by metabolomics: a plant with potential benefit for management of diabetes in traditional Chinese medicine[J]. METABOLOMICS,2019,15(8):104. |
| APA | Zhou, Shuaizhen.,Allard, Pierre-Marie.,Wolfrum, Christian.,Ke, Changqiang.,Tang, Chunping.,...&Wolfender, Jean-Luc.(2019).Identification of chemotypes in bitter melon by metabolomics: a plant with potential benefit for management of diabetes in traditional Chinese medicine.METABOLOMICS,15(8),104. |
| MLA | Zhou, Shuaizhen,et al."Identification of chemotypes in bitter melon by metabolomics: a plant with potential benefit for management of diabetes in traditional Chinese medicine".METABOLOMICS 15.8(2019):104. |
入库方式： OAI收割
来源：上海药物研究所
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