Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly
文献类型：期刊论文
| 作者 | Yan, Runqi1; Hu, Yuxuan1; Liu, Fei1; Wei, Shixuan1; Fang, Daqing2,3; Shuhendler, Adam J.4; Liu, Hong2,3 ; Chen, Hong-Yuan1; Ye, Deju1,5
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| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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| 出版日期 | 2019-07-03 |
| 卷号 | 141期号:26页码:10331-10341 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.9b03649 |
| 文献子类 | Article |
| 英文摘要 | Stimuli-responsive in situ self-assembly of small molecules to form nanostructures in living subjects has produced promising tools for molecular imaging and tissue engineering. However, controlling the self-assembly process to simultaneously activate multimodality imaging signals in a small-molecule probe is challenging. In this paper, we rationally integrate a fluorogenic reaction into enzyme-responsive in situ self-assembly to design small-molecule-based activatable near infrared (NIR) fluorescence and magnetic resonance (MR) bimodal probes for molecular imaging. Using alkaline phosphatase (ALP) as a model target, we demonstrate that probe (P-CyFF-Gd) can be activated by endogenous ALP overexpressed on cell membranes, producing membrane-localized assembled nanoparticles (NPs) that can be directly visualized by cryo-SEM. Simultaneous enhancements in NIR fluorescence (>70-fold at 710 nm) and r(1) relaxivity (similar to 2.3-fold) enable real-time, high-sensitivity, high-spatial-resolution imaging and localization of the ALP activity in live tumor cells and mice. P-CyFF-Gd can also delineate orthotopic liver tumor foci, facilitating efficient real-time, image-guided surgical resection of tumor tissues in intraoperative mice. This strategy combines activatable NIR fluorescence via a fluorogenic reaction and activatable MRI via in situ self-assembly to promote ALP activity imaging, which could be applicable to design other activatable bimodal probes for in vivo imaging of enzyme activity and locations in real time. |
| WOS关键词 | INTESTINAL ALKALINE-PHOSPHATASE ; CONTRAST AGENT ; CANCER ; LIVER ; NANOPARTICLES ; NANOFIBERS ; APOPTOSIS ; SURGERY ; SIGNAL ; TUMORS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000474669700027 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309427]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Ye, Deju |
| 作者单位 | 1.Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, S55 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, S55 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China; 4.Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON K1N 6N5, Canada; 5.Nanjing Univ, Res Ctr Environm Nanotechnol ReCent, Nanjing 210023, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Runqi,Hu, Yuxuan,Liu, Fei,et al. Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(26):10331-10341. |
| APA | Yan, Runqi.,Hu, Yuxuan.,Liu, Fei.,Wei, Shixuan.,Fang, Daqing.,...&Ye, Deju.(2019).Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(26),10331-10341. |
| MLA | Yan, Runqi,et al."Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.26(2019):10331-10341. |
入库方式： OAI收割
来源：上海药物研究所
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