Single-base mapping of m6A by an antibody-independent method
文献类型：期刊论文
| 作者 | Zhang, Zhang1; Chen, Li-Qian1; Zhao, Yu-Li1; Yang, Cai-Guang2 ; Roundtree, Ian A.3,4; Zhang, Zijie3,4; Ren, Jian1; Xie, Wei1; He, Chuan3,4; Luo, Guan-Zheng1
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| 刊名 | SCIENCE ADVANCES
 |
| 出版日期 | 2019-07-03 |
| 卷号 | 5期号:7页码:eaax0250 |
| ISSN号 | 2375-2548 |
| DOI | 10.1126/sciadv.aax0250 |
| 文献子类 | Article |
| 英文摘要 | N-6-methyladenosine (m(6)A) is one of the most abundant messenger RNA modifications in eukaryotes involved in various pivotal processes of RNA metabolism. The most popular high-throughput m(6)A identification method depends on the anti-m(6)A antibody but suffers from poor reproducibility and limited resolution. Exact location information is of great value for understanding the dynamics, machinery, and functions of m(6)A. Here, we developed a precise and high-throughput antibody-independent m(6)A identification method based on the m(6)A-sensitive RNA endoribonuclease recognizing ACA motif (m(6)A-sensitive RNA-Endoribonuclease-Facilitated sequencing or m(6)A-REF-seq). Whole-transcriptomic, single-base m(6)A maps generated by m(6)A-REF-seq quantitatively displayed an explicit distribution pattern with enrichment near stop codons. We used independent methods to validate methylation status and abundance of individual m(6)A sites, confirming the high reliability and accuracy of m(6)A-REF-seq. We applied this method on five tissues from human, mouse, and rat, showing that m(6)A sites are conserved with single-nucleotide specificity and tend to cluster among species. |
| WOS关键词 | MESSENGER-RNA ; N-6-METHYLADENOSINE RNA ; NUCLEOTIDE-RESOLUTION ; METHYLATION ; REVEALS ; LEUKEMIA |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000478770400107 |
| 出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309429]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Luo, Guan-Zheng |
| 作者单位 | 1.Sun Yat Sen Univ, Sch Life Sci, MOE Key Lab Gene Funct & Regulat, State Key Lab Biocontrol, Guangzhou, Guangdong, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Chem Biol, State Key Lab Drug Res, Shanghai, Peoples R China; 3.Univ Chicago, Dept Chem, Inst Biophys Dynam, Howard Hughes Med Inst, 5735 S Ellis Ave, Chicago, IL 60637 USA; 4.Univ Chicago, Dept Biochem & Mol Biol, Inst Biophys Dynam, Howard Hughes Med Inst, 920 E 58Th St, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Zhang,Chen, Li-Qian,Zhao, Yu-Li,et al. Single-base mapping of m6A by an antibody-independent method[J]. SCIENCE ADVANCES,2019,5(7):eaax0250. |
| APA | Zhang, Zhang.,Chen, Li-Qian.,Zhao, Yu-Li.,Yang, Cai-Guang.,Roundtree, Ian A..,...&Luo, Guan-Zheng.(2019).Single-base mapping of m6A by an antibody-independent method.SCIENCE ADVANCES,5(7),eaax0250. |
| MLA | Zhang, Zhang,et al."Single-base mapping of m6A by an antibody-independent method".SCIENCE ADVANCES 5.7(2019):eaax0250. |
入库方式： OAI收割
来源：上海药物研究所
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