Fluorescent Triazole Urea Activity-Based Probes for the Single-Cell Phenotypic Characterization of Staphylococcus aureus
文献类型：期刊论文
| 作者 | Chen, Linhai1,2 ; Keller, Laura J.3; Cordasco, Edward1; Bogyo, Matthew1; Lentz, Christian S.1,4
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| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2019-04-16 |
| 卷号 | 58期号:17页码:5643-5647 |
| 关键词 | activity-based probes enzymes molecular imaging protein profiling single-cell analysis |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201900511 |
| 文献子类 | Article |
| 英文摘要 | Phenotypically distinct cellular (sub) populations are clinically relevant for the virulence and antibiotic resistance of a bacterial pathogen, but functionally different cells are usually indistinguishable from each other. Herein, we introduce fluorescent activity-based probes as chemical tools for the single-cell phenotypic characterization of enzyme activity levels in Staphylococcus aureus. We screened a 1,2,3-triazole urea library to identify selective inhibitors of fluorophosphonate-binding serine hydrolases and lipases in S. aureus and synthesized target-selective activity-based probes. Molecular imaging and activity-based protein profiling studies with these probes revealed a dynamic network within this enzyme family involving compensatory regulation of specific family members and exposed single-cell phenotypic heterogeneity. We propose the labeling of enzymatic activities by chemical probes as a generalizable method for the phenotyping of bacterial cells at the population and single-cell level. |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000466593400025 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309433]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Bogyo, Matthew; Lentz, Christian S. |
| 作者单位 | 1.Stanford Univ, Sch Med, Dept Pathol, 300 Pasteur Dr, Stanford, CA 94305 USA; 2.Chinese Acad Sci, Shanghai Inst Mat Medica, State Key Lab Drug Res, Natl Ctr Drug Screening, 189 Guoshoujing Rd, Shanghai 201203, Peoples R China; 3.Stanford Univ, Sch Med, Dept Chem & Syst Biol, 300 Pasteur Dr, Stanford, CA 94305 USA; 4.Helmholtz Ctr Infect Res HZI, Dept Chem Biol, Braunschweig, Germany |
| 推荐引用方式 GB/T 7714 | Chen, Linhai,Keller, Laura J.,Cordasco, Edward,et al. Fluorescent Triazole Urea Activity-Based Probes for the Single-Cell Phenotypic Characterization of Staphylococcus aureus[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2019,58(17):5643-5647. |
| APA | Chen, Linhai,Keller, Laura J.,Cordasco, Edward,Bogyo, Matthew,&Lentz, Christian S..(2019).Fluorescent Triazole Urea Activity-Based Probes for the Single-Cell Phenotypic Characterization of Staphylococcus aureus.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,58(17),5643-5647. |
| MLA | Chen, Linhai,et al."Fluorescent Triazole Urea Activity-Based Probes for the Single-Cell Phenotypic Characterization of Staphylococcus aureus".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 58.17(2019):5643-5647. |
入库方式： OAI收割
来源：上海药物研究所
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