A hydrogen-deuterium exchange mass spectrometry-based protocol for protein-small molecule interaction analysis
文献类型：期刊论文
| 作者 | Meng, Qian1,2; Song, Yuan-Li1,3; Zhou, Chen1; He, Han1; Zhang, Naixia1,3 ; Zhou, Hu1,3,4
|
| 刊名 | Biophysics reports
 |
| 出版日期 | 2023-04-30 |
| 卷号 | 9期号:2页码:99-111 |
| ISSN号 | 2364-3420 |
| DOI | 10.52601/bpr.2023.230006 |
| 文献子类 | Article |
| 英文摘要 | Protein-small molecule interaction is vital in regulating protein functions and controlling various cellular processes. Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful methodology to study protein-small molecule interactions, however, to accurately probe the conformational dynamics of the protein upon small molecule binding, the HDX-MS experimental conditions should be carefully controlled and optimized. Here, we present the detailed continuous-labeling, bottom-up HDX-MS protocol for studying protein-small molecule interactions. We took a side-by-side HDX kinetics comparison of the Hsp90N protein with or without the treatment of small molecules (i.e., Radicicol, Geldanamycin) for displaying conformational changes induced by molecular interactions between Hsp90N and small molecules. Our sensitive and robust experimental protocol can facilitate the novice to quickly carry out the structural characterization of protein-small molecule interactions. |
| WOS关键词 | Hsp90N ; Hydrogen-deuterium exchange mass spectrometry (HDX-MS) ; Protein dynamics ; Protein-small molecule interactions |
| 语种 | 英语 |
| WOS记录号 | MEDLINE: 37753061 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309616]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zhang, Naixia; Zhou, Hu |
| 作者单位 | 1.Analytical Research Center for Organic and Biological Molecules, State Key Laboratory of Drug Research, State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Department of Pharmacology, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. 3.University of Chinese Academy of Sciences, Beijing 100049, China. 4.School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. |
| 推荐引用方式 GB/T 7714 | Meng, Qian,Song, Yuan-Li,Zhou, Chen,et al. A hydrogen-deuterium exchange mass spectrometry-based protocol for protein-small molecule interaction analysis[J]. Biophysics reports,2023,9(2):99-111. |
| APA | Meng, Qian,Song, Yuan-Li,Zhou, Chen,He, Han,Zhang, Naixia,&Zhou, Hu.(2023).A hydrogen-deuterium exchange mass spectrometry-based protocol for protein-small molecule interaction analysis.Biophysics reports,9(2),99-111. |
| MLA | Meng, Qian,et al."A hydrogen-deuterium exchange mass spectrometry-based protocol for protein-small molecule interaction analysis".Biophysics reports 9.2(2023):99-111. |
入库方式： OAI收割
来源：上海药物研究所
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