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Tepidibacter hydrothermalis sp. nov., a novel anaerobic bacterium isolated from a deep-sea hydrothermal vent

文献类型：期刊论文

作者Dai, Jie6; Li, Xue-Gong1,2,3,4,5; Zhang, Wei-Jia1,2,3,4,5; Wu, Long-Fei3,4,6
刊名INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
出版日期2023
卷号73期号:11页码:8
ISSN号1466-5026
关键词anaerobe Tepidibacter hydrothermalis hydrothermal vent Southwest Indian Ridge hydrogenase tat export system
DOI10.1099/ijsem.0.006151
英文摘要A novel anaerobic heterotrophic bacterium, designated strain SWIR-1(T), was isolated from a deep-sea hydrothermal vent field sample collected from the Southwest Indian Ridge at a depth of 2700 m. Phylogenetic analysis indicated that strain SWIR-1(T) belongs to the genus Tepidibacter, and the most closely related species are Tepidibacter mesophilus B1(T) (99.1 % 16S rRNA gene sequence similarity), Tepidibacter formicigenes DV1184(T) (94.6%) and Tepidibacter thalassicus SC562(T) (93.9 %). Strain SWIR-1T shares 77.3-87.2% average nucleotide identity and 21.5-35.7% digital DNA-DNA hybridization values with the three type strains of Tepidibacter species. Cells of strain SWIR-1(T) were Gram-stain- positive, motile, short straight rods. Endospores were observed in stationary-phase cells when grown on Thermococcales rich medium. Strain SWIR-1(T) grew at 15-45 degrees C (optimum, 30 degrees C), at pH 5.5-8.0 (optimum, pH 7.0) and with 1.0-6.0% (w/v) NaCl (optimum, 2.0%). Substrates utilized by strain SWIR-1(T) included complex proteinaceous, chitin, starch, lactose, maltose, fructose, galactose, glucose, rhamnose, arabinose, ribose, alanine, glycine and glycerol. The major fermentation products from glucose were acetate, lactate, H-2 and CO2. Elemental sulphur, sulphate, thiosulphate, sulphite, fumarate, nitrate, nitrite and FeCl3 are not used as terminal electron acceptors. The main cellular fatty acids consisted of iso-C-15:0 (28.4%), C-15:1 iso F (15.4%) and C-16: 0 (9.8%). The major polar lipids were phospholipids and glycolipids. No respiratory quinones were detected. Genomic comparison revealed a distinctive blended gene cluster comprising hyb-tat- hyp genes, which play a crucial role in the synthesis, maturation, activation and export of NiFe-hydrogenase. Based on the phylogenetic analysis, genomic, physiologic and chemotaxonomic characteristics, strain SWIR-1T is considered to represent a novel species within the genus Tepidibacter, for which the name Tepidibacter hydrothermalis sp. nov. is proposed. The type strain is strain SWIR-1(T) (=DSM 113848(T)=MCCC 1K07078(T))
WOS关键词MICROBES ; SUBUNIT
资助项目Major Scientific and Technological Projects of Hainan Province[ZDKJ2021028] ; Major Scientific and Technological Projects of Hainan Province[ZDKJ2019011] ; National Natural Science Foundation of China[NSFC 42176121] ; National Natural Science Foundation of China[42 076 127] ; Centre National de la Recherche Scientifique
WOS研究方向Microbiology
语种英语
出版者MICROBIOLOGY SOC
WOS记录号WOS:001140942400032
资助机构Major Scientific and Technological Projects of Hainan Province ; National Natural Science Foundation of China ; Centre National de la Recherche Scientifique
源URL[http://ir.idsse.ac.cn/handle/183446/10810]  
专题深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
通讯作者Li, Xue-Gong; Wu, Long-Fei
作者单位1.Hainan Deep Sea Technol Lab, Inst Deep Sea Life Sci, IDSSE BGI, Sanya, Hainan, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Expt Study Deep Sea Extreme Condit, Inst Deep Sea Sci & Engn, Sanya, Peoples R China
3.Chinese Acad Sci, Sanya, Peoples R China
4.CNRS, Int Associated Lab Evolut & Dev Magnetotact Multi, Marseille, France
5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya, Peoples R China
6.Aix Marseille Univ, CNRS, LCB, IMM,IM2B, Marseille, France
推荐引用方式
GB/T 7714		 Dai, Jie,Li, Xue-Gong,Zhang, Wei-Jia,et al. Tepidibacter hydrothermalis sp. nov., a novel anaerobic bacterium isolated from a deep-sea hydrothermal vent[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2023,73(11):8.
APA Dai, Jie,Li, Xue-Gong,Zhang, Wei-Jia,&Wu, Long-Fei.(2023).Tepidibacter hydrothermalis sp. nov., a novel anaerobic bacterium isolated from a deep-sea hydrothermal vent.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,73(11),8.
MLA Dai, Jie,et al."Tepidibacter hydrothermalis sp. nov., a novel anaerobic bacterium isolated from a deep-sea hydrothermal vent".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 73.11(2023):8.

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来源：深海科学与工程研究所

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