Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction
文献类型：期刊论文
| 作者 | Xu, Peikuan2; Yang, Ming1 ; He, Lisheng1; Zhang, Hongxi1; Gao, Zhaoming1; Jiang, Yuelu2; Wang, Yong2; Antonarakou, Assimina
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| 刊名 | JOURNAL OF MARINE SCIENCE AND ENGINEERING
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| 出版日期 | 2024 |
| 卷号 | 12期号:1页码:12 |
| 关键词 | eukaryotic microbe MISNAC Retaria South China Sea Niskin |
| DOI | 10.3390/jmse12010061 |
| 英文摘要 | Deep-sea eukaryotic microorganisms play a vital role in degrading organic matter and geochemically cycling elements in the deep ocean. However, the impact of sampling methods on detection of these microorganisms under high hydrostatic pressure remains uncertain. In this study, we compared a traditional water sampling method using a Niskin bottle, an in situ microbial filtration and fixation method (ISMIFF), and a multiple in situ nucleic acid collection (MISNAC) method to exhibit differences in the community structures that were collected at similar to 590-3100 m in the South China Sea (SCS). The classification and biodiversity indices of 18S rDNA Illumina sequencing reads from the V9 variation region revealed higher diversity for MISNAC DNA absorption column samples compared to others. Importantly, the relative abundance of Ciliophora (19.49%), Copepoda (23.31%), and Diplonemea (10.67%) was higher in MISNAC adsorption column samples, while Retaria (48.86%) were dominant in the MISNAC membrane samples. This indicates that MISNAC columns might collect more DNA in situ for the naked protists, while Retaria with a carbonate shell were more likely retained on the membrane. In conclusion, MISNAC is an effective method for DNA collection of deep-sea eukaryotic microorganisms and provides valuable materials for studying deep-sea microbial ecosystems. |
| WOS关键词 | MICROBIAL EUKARYOTES ; SEQUENCES ; TOOL |
| 资助项目 | Hainan Provincial Natural Science Foundation of China ; Supercomputer Center of Sanya University |
| WOS研究方向 | Engineering ; Oceanography |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:001152719100001 |
| 资助机构 | Hainan Provincial Natural Science Foundation of China ; Supercomputer Center of Sanya University |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/10845]  |
| 专题 | 深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组 深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组 |
| 通讯作者 | Jiang, Yuelu; Wang, Yong |
| 作者单位 | 1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 2.Tsinghua Univ, Inst Ocean Engn, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Peikuan,Yang, Ming,He, Lisheng,et al. Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2024,12(1):12. |
| APA | Xu, Peikuan.,Yang, Ming.,He, Lisheng.,Zhang, Hongxi.,Gao, Zhaoming.,...&Antonarakou, Assimina.(2024).Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction.JOURNAL OF MARINE SCIENCE AND ENGINEERING,12(1),12. |
| MLA | Xu, Peikuan,et al."Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction".JOURNAL OF MARINE SCIENCE AND ENGINEERING 12.1(2024):12. |
入库方式： OAI收割
来源：深海科学与工程研究所
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