Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Men, Jia L.1; Zhang, Yu T.1; Pei, Yue B.1; Li, Na1; Xiang, Jian H.2; Zhou, Hai L.1,3,4 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2024-02-25 |
| 卷号 | 581页码:14 |
| ISSN号 | 0044-8486 |
| 关键词 | Litopenaeus vannamei Nanocarrier CRISPR/Cas9 gene editing Transfection Early embryos |
| DOI | 10.1016/j.aquaculture.2023.740424 |
| 通讯作者 | Zhou, Hai L.(zhouhl@hainanu.edu.cn) |
| 英文摘要 | Cultivation of superior varieties is the key to maintaining the sustainable development of the Litopenaeus van-namei (L.vannamei) industry. CRISPR/Cas9 technology represents a generation of genetic breeding technology based on gene editing. However, the conventional delivery strategy of CRISPR/Cas9 components could not be used due to the particular physiological traits and practical difficulties of L. vannamei embryos. We designed and established polyethylenimine (PEI)-coated nanoparticles with carboxylated SNWTs core to safely deliver CRISPR/Cas9 plasmids into early embryos for target gene editing. The results showed that the transfection ef-ficiency of this strategy was 36%, which was approximately 4-fold higher than the efficiency of the classical lipid transfection method. The transcription factor Pax6, which has notable effects on early embryonic eye develop-ment, provides clear phenotypic proof for this strategy. Unnatural base alterations were found in up to 20% of transfected embryos. This study establishes a foundation for the application of CRISPR technology in L. vannamei and provides an innovative approach for large-scale gene function studies in aquaculture. |
| WOS关键词 | HATCHING ENVELOPE FORMATION ; DETERMINATION GENE NETWORK ; BLACK TIGER SHRIMP ; CARBON NANOTUBES ; PENAEUS-MONODON ; IN-VITRO ; DNA ; BACTERIA ; PAX6 ; POLYETHYLENIMINE |
| 资助项目 | Hainan Province Science and Technology Special Fund[ZDYF2022SHFZ321] ; Graduate Innovation Research Project of Hainan Province[Qhyb2022-44] ; Hainan Basic and Applied Basic Research Program (Natural Sciences) (High-level Talents Project)[2019RC077] ; National Natural Science Foundation of China[42377273] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:001139843700001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184290]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Zhou, Hai L. |
| 作者单位 | 1.Hainan Univ, Sch Life Sci, Haikou 570228, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Hainan Univ, One Hlth Inst, Haikou 570228, Hainan, Peoples R China 4.Hainan Univ, State Key Lab South China Sea Marine Resource Util, Haikou 570228, Peoples R China |
| 推荐引用方式 GB/T 7714 | Men, Jia L.,Zhang, Yu T.,Pei, Yue B.,et al. Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei[J]. AQUACULTURE,2024,581:14. |
| APA | Men, Jia L.,Zhang, Yu T.,Pei, Yue B.,Li, Na,Xiang, Jian H.,&Zhou, Hai L..(2024).Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei.AQUACULTURE,581,14. |
| MLA | Men, Jia L.,et al."Development of a PEI-coated SWNTs Nanocarrier for efficient delivery of CRISPR/Cas9 in early embryos of Litopenaeus vannamei".AQUACULTURE 581(2024):14. |
入库方式： OAI收割
来源：海洋研究所
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