Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen
文献类型：期刊论文
| 作者 | Ni, Qian3; Liang, Xiafei3; Yang, Shengyi3; Ge, Hongxing2,3,4; Dong, Zhiguo1,3,4 |
| 刊名 | AQUACULTURE REPORTS
 |
| 出版日期 | 2024-04-01 |
| 卷号 | 35页码:9 |
| ISSN号 | 2352-5134 |
| 关键词 | CsRhbg GSase GDH activity Cyclina sinensis Chronic ammonia nitrogen exposure |
| DOI | 10.1016/j.aqrep.2024.101952 |
| 通讯作者 | Ge, Hongxing(hongxinggeliu@163.com) ; Dong, Zhiguo(dzg7712@163.com) |
| 英文摘要 | The clam Cyclina sinensis is characterized by good ammonia resistance. With the purpose of gaining knowledge about its ammonia resistance, we assessed the mRNA expression levels of Rhesus glucoprotein b (CsRhbg), the activities of key enzymes in the ammonia transport pathways (glutamine synthetase, GS; glutamic acid dehydrogenase, GDH) in the gill and hepatopancreas and the gill histopathological section (HE staining) of the clam exposed to total ammonia nitrogen (TAN) (6.58 mg/L) for 768 h (32d). Results revealed that the full-length cDNA sequence of CsRhbg was 1777 bp in length. The CsRhbg was highly conserved in the same genus, and the protein encoded by CsRhbg contains 10 transmembrane structures (TMHs) and one signal peptide. Exposed to ammonia nitrogen, the expression level of CsRhbg mRNA climbed sharply at 3 h post-exposure, and then it decreased and fluctuated from 192 h to 768 h. The activity of GSase in the clam exposed to ammonia nitrogen increased in the first 24 h, and reached the highest value at 96 h, and then fluctuated. The activity of GDH rose sharply and reached the highest value at 3 h, and then fluctuated. With the increase of exposure time, histological alterations in the gill became more and more clear in the disappearance of the epithelial cell boundaries. The results suggest that exposure to ammonia nitrogen can activate CsRhbg gene expression, and elevate the GSase and GDH activity in the gill of the clam as the ammonia detoxification strategy to reduce the harm of ammonia. However, prolonged exposure to ammonia nitrogen can result in the down-regulation of CsRhbg gene, dysfunction of key enzymes in the ammonia transport pathways, and damage to the structure of epithelial cells or the cellular structure in the gill of the clam. Therefore, this work will help to reveal the ammonia detoxification strategy in bivalves exposed to chronic ammonia nitrogen. |
| WOS关键词 | RAINBOW-TROUT ; GLUTAMINE-SYNTHETASE ; RHESUS GLYCOPROTEINS ; SUBLETHAL AMMONIA ; EXPRESSION ; BRAIN ; INHIBITION ; METABOLISM ; EXCRETION ; FAMILY |
| 资助项目 | China Agriculture Research System of MOF ; Postdoctoral Fellowship of Lianyungang[SH20211209] ; Opening Foundation of Jiangsu Key Laboratory of Marine Bioresources and Environment[JSIMR202017] ; Opening Foundation of Jiangsu Institute of Marine Resources Development[JBGS [2021] 034] ; JBGS Project of Seed Industry Revitalization in Jiangsu Province[CARS-49] ; China Agriculture Research System of MARA ; Priority Academic Program Development of Jiangsu ; [LYG20210008] |
| WOS研究方向 | Fisheries |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:001173451900001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184577]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Ge, Hongxing; Dong, Zhiguo |
| 作者单位 | 1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 3.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222005, Jiangsu, Peoples R China 4.Jiangsu Marine Resources Dev Inst, Lianyungang 222005, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ni, Qian,Liang, Xiafei,Yang, Shengyi,et al. Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen[J]. AQUACULTURE REPORTS,2024,35:9. |
| APA | Ni, Qian,Liang, Xiafei,Yang, Shengyi,Ge, Hongxing,&Dong, Zhiguo.(2024).Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen.AQUACULTURE REPORTS,35,9. |
| MLA | Ni, Qian,et al."Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen".AQUACULTURE REPORTS 35(2024):9. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。