Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture
文献类型：期刊论文
| 作者 | Duan, Lei1,2,3; Du, Shuran1,3; Wang, Xueying1,3 ; Zhou, Li1,3; Liu, Qinghua1,3; Li, Jun1,3
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| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2024-01-15 |
| 卷号 | 214页码:1-9 |
| ISSN号 | 0093-691X |
| 关键词 | GDNF Germ cells Germ cell transplantation Turbot |
| DOI | 10.1016/j.theriogenology.2023.09.013 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | Germ cell transplantation (GCT) is a promising biotechnology that enables the production of donor-derived gametes in surrogate recipients. It plays a crucial role in the protection of endangered species, the propaga-tion of elite species with desired traits, and long-term preservation of genetic resources. This significance is particularly pronounced when GCT is synergistically employed with cryopreservation techniques. However, GCT often encounters challenges due to low colonization rates and, in some cases, complete loss of donor cells in recipients. Glial cell line-derived neurotrophic factor (GDNF) plays a pivotal role in sustaining the self-renewal of spermatogonial stem cells (SSCs) in mammals. Additionally, it has been shown to promote the proliferation of spermatogonia in vitro cultures in certain animal species. In turbot (Scophthalmus maximus), we found that the expressions of gdnf and gfr alpha 1a were predominantly observed in spermatogonia rather than somatic cells, which differed from their expression patterns in mammals. The efficiency of exogenous spermatogonia transplantation in Japanese flounder (Paralichthys olivaceus) larvae could be substantially enhanced by incubating donor cells from turbot with 100 ng/ml GDNF prior to transplantation. This led to a noteworthy increase in the colonization rate, rising from 33%-50%-61.5%. Additionally, the addition of 20 ng/ml GDNF in cell medium could also promote the proliferation of turbot germ cells in vitro. These results demonstrated the gdnf in turbot testis expression characteristics and suggested that addition of GNDF could be an effective way to improve the GCT efficiency and promote the germ cells expansion during in vitro culture. |
| WOS关键词 | SPERMATOGONIAL STEM-CELLS ; YELLOWTAIL SERIOLA-QUINQUERADIATA ; RAINBOW-TROUT ; PROLIFERATION ; TRANSPLANTATION ; MAINTENANCE ; GENERATION ; SURROGATE ; SURVIVAL ; TESTIS |
| 资助项目 | Shandong Province Natural Science Foundation[ZR2020KC038] ; Research and Development Program of Shandong Province[2021LZGC029] ; China Agriculture Research System[CARS-47] |
| WOS研究方向 | Reproductive Biology ; Veterinary Sciences |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE INC |
| WOS记录号 | WOS:001098779600001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/183849]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Lei,Du, Shuran,Wang, Xueying,et al. Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture[J]. THERIOGENOLOGY,2024,214:1-9. |
| APA | Duan, Lei,Du, Shuran,Wang, Xueying,Zhou, Li,Liu, Qinghua,&Li, Jun.(2024).Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture.THERIOGENOLOGY,214,1-9. |
| MLA | Duan, Lei,et al."Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture".THERIOGENOLOGY 214(2024):1-9. |
入库方式： OAI收割
来源：海洋研究所
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