Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia
文献类型：期刊论文
| 作者 | Duan, Hu1,5; Shao, Xuanxuan5; Liu, Wei5; Xiang, Jianhai2 ; Pan, Namin5; Wang, Xuehui4; Du, Guoru5; Li, Ying5; Zhou, Jiaping3; Sui, Liying1,5
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| 刊名 | OPEN BIOLOGY
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| 出版日期 | 2023-11-15 |
| 卷号 | 13期号:11页码:14 |
| 关键词 | ovarian development oogenesis germline stem cell parthenogenetic Artemia RNA-seq Ap-VgR function |
| DOI | 10.1098/rsob.230172 |
| 通讯作者 | Sui, Liying(suily@tust.edu.cn) |
| 英文摘要 | The halophilic zooplankton brine shrimp Artemia has been used as an experimental animal in multidisciplinary studies. However, the reproductive patterns and its regulatory mechanisms in Artemia remain unclear. In this study, the ovarian development process of parthenogenetic Artemia (A. parthenogenetica) was divided into five stages, and oogenesis or egg formation was identified in six phases. The oogenesis mode was assumed to be polytrophic. We also traced the dynamic translocation of candidate germline stem cells (cGSCs) using EdU labelling and elucidated several key cytological events in oogenesis through haematoxylin and eosin staining and fluorescence imaging. Distinguished from the ovary structure of insects and crustaceans, Artemia germarium originated from ovariole buds and are located at the base of the ovarioles. RNA-seq based on five stages of ovarian development identified 2657 upregulated genes related to reproduction by pair-to-pair comparison. Gbb, Dpp, piwi, vasa, nanos, VgA and VgR genes associated with cGSCs recognition and reproductive development were screened and verified using qPCR. Silencing of the VgR gene in A. parthenogenetica (Ap-VgR) at ovarian development Stage II led to a low level of gene expression (less than 10%) within 5 days, which resulted in variations in oogenesis-related gene expression and significantly inhibited vitellogenesis, impeded oocyte maturation, and eventually decreased the number of offspring. In conclusion, we have illustrated the patterns of ovarian development, outlined the key spatio-temporal features of oogenesis and identified the negative impacts of VgR gene knockdown on oogenesis using A. parthenogenetica as an experimental animal. The findings of this study also lay a foundation for the further study of reproductive biology of invertebrates. |
| WOS关键词 | VITELLOGENIN RECEPTOR ; MOLECULAR CHARACTERIZATION ; EXPRESSION ; SHRIMP ; REPRODUCTION ; MAINTENANCE ; OOGENESIS ; EVOLUTION ; INSIGHT ; CELLS |
| 资助项目 | We would like to thank Dr Zengyu Ma for her help with qPCR and RNAi assays. |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | ROYAL SOC |
| WOS记录号 | WOS:001105039900001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/183897]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sui, Liying |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Key Lab Marine Resource Chem & Food Technol, Minist Educ, 29,13th Ave, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Tianjin Univ Sci & Technol, Res Ctr Modern Analyt Technol, 29,13th Ave, Tianjin 300457, Peoples R China 4.Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China 5.Tianjin Univ Sci & Technol, Coll Marine & Environm Sci, Asian Reg Artemia Reference Ctr, 29,13th Ave, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Hu,Shao, Xuanxuan,Liu, Wei,et al. Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia[J]. OPEN BIOLOGY,2023,13(11):14. |
| APA | Duan, Hu.,Shao, Xuanxuan.,Liu, Wei.,Xiang, Jianhai.,Pan, Namin.,...&Sui, Liying.(2023).Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia.OPEN BIOLOGY,13(11),14. |
| MLA | Duan, Hu,et al."Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia".OPEN BIOLOGY 13.11(2023):14. |
入库方式： OAI收割
来源：海洋研究所
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