Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity
文献类型：期刊论文
| 作者 | Hao, Kangwei1,2,3; Xu, Hang1,2,3 ; Jiang, Shuai1,2,3; Sun, Li1,2,3
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| 刊名 | FRONTIERS IN IMMUNOLOGY
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| 出版日期 | 2024-01-16 |
| 卷号 | 15页码:11 |
| 关键词 | Paralichthys olivaceus necroptosis RIPK1 RIPK3 MLKL immune defense |
| ISSN号 | 1664-3224 |
| DOI | 10.3389/fimmu.2024.1348866 |
| 通讯作者 | Jiang, Shuai(sjiang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Necroptosis is a type of proinflammatory programmed necrosis essential for innate immunity. The receptor interacting protein kinases 1/3 (RIPK1/3) and the substrate mixed lineage kinase domain-like protein (MLKL) are core components of the necroptotic axis. The activation and immunological function of necroptosis in fish remain elusive. Herein, we studied the function and activation of RIPK1/3 (PoRIPK1/3) and MLKL (PoMLKL) in teleost Paralichthys olivaceus. Bacterial infection increased the expression of RIPK1/3 and MLKL. The N-terminal four-helix bundle (4HB) domain of PoMLKL exhibited necroptosis-inducing activity, and the C-terminal pseudokinase domain exerted auto-inhibitory effect on the 4HB domain. PoRIPK3 was capable of phosphorylating the T360/S361 residues in the PoMLKL C-terminal domain and initiated necroptosis, and this necroptosis-inducing activity was enhanced by PoRIPK1. PoRIPK1/3 interacted with PoMLKL in a manner that depended on the RIP homotypic interaction motif (RHIM), and deletion of RHIM from PoRIPK1/3 led to the dissociation of PoRIPK1/3 with PoMLKL. Inhibition of PoMLKL-mediated necroptosis increased Edwardsiella tarda infection in fish cells and tissues, and led to significantly enhanced lethality of the host. Taken together, these results revealed the activation mechanism of PoRIPK1/3-PoMLKL signaling pathway and the immunological function of necroptosis in the immune defense of teleost. |
| WOS关键词 | MIXED LINEAGE KINASE ; DOMAIN-LIKE ; MOLECULAR-PATTERNS ; RELEASE ; PHOSPHORYLATION ; DOWNSTREAM ; NECROSIS |
| 资助项目 | Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2020Q03] ; Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000] ; Taishan Scholar Program of Shandong Province[2018] ; Taishan Scholar Program of Shandong Province[2021] ; Youth Innovation Promotion Association CAS[2021204] |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:001153555100001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184404]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Jiang, Shuai; Sun, Li |
| 作者单位 | 1.Lab Marine Biol & Biotechnol, Laoshan Lab, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Megasci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Kangwei,Xu, Hang,Jiang, Shuai,et al. Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity[J]. FRONTIERS IN IMMUNOLOGY,2024,15:11. |
| APA | Hao, Kangwei,Xu, Hang,Jiang, Shuai,&Sun, Li.(2024).Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity.FRONTIERS IN IMMUNOLOGY,15,11. |
| MLA | Hao, Kangwei,et al."Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity".FRONTIERS IN IMMUNOLOGY 15(2024):11. |
入库方式： OAI收割
来源：海洋研究所
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