A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates
文献类型：期刊论文
| 作者 | Gu, Xiaoqian1,4; Fu, Liping4; Wang, Zhiyan4; Cao, Zhe4; Zhao, Luying4; Seswita-Zilda, Dewi3; Zhang, Ao2; Zhang, Qian4; Li, Jiang4 |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2024-02-19 |
| 卷号 | 72期号:8页码:4116-4126 |
| ISSN号 | 0021-8561 |
| 关键词 | bifunctional alginatelyase alginate oligosaccharides antioxidant activity |
| DOI | 10.1021/acs.jafc.3c08638 |
| 通讯作者 | Li, Jiang(lijiang@fio.org.cn) |
| 英文摘要 | Alginate lyase Aly448, a potential new member of the polysaccharide lyase (PL) 7 family, which was cloned and identified from the macroalgae-associated bacterial metagenomic library, showed bifunctionality. The molecular docking results revealed that Aly448 has two completely different binding sites for alginate (polyMG), poly-alpha-l-guluronic acid (polyG), and poly-beta-d-mannuronic acid (polyM) substrates, respectively, which might be the molecular basis for the enzyme's bifunctionality. Truncational results confirmed that predicted key residues affected the bifunctionality of Aly448, but did not wholly explain. Besides, Aly448 presented excellent biochemical characteristics, such as higher thermal stability and pH tolerance. Degradation of polyMG, polyM, and polyG substrates by Aly448 produced tetrasaccharide (DP4), disaccharide (DP2), and galactose (DP1), which exhibited excellent antioxidant activity. These findings provide novel insights into the substrate recognition mechanism of bifunctional alginate lyases and pave a new path for the exploitation of natural antioxidant agents. |
| WOS关键词 | PURIFICATION ; OLIGOSACCHARIDES ; PL-7 |
| 资助项目 | Impact and Response of Antarctic Seas to Climate Change[IRFSOCC2023-2025] ; Impact and Response of Antarctic Seas to Climate Change |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:001166898100001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184487]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Jiang |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Chem Engn Inst, Qingdao 266042, Peoples R China 3.Natl Res & Innovat Agcy BRIN, Res Ctr Deep Sea Earth Sci & Maritime Res Org, Jakarta 14430, Indonesia 4.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao 266061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Xiaoqian,Fu, Liping,Wang, Zhiyan,et al. A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(8):4116-4126. |
| APA | Gu, Xiaoqian.,Fu, Liping.,Wang, Zhiyan.,Cao, Zhe.,Zhao, Luying.,...&Li, Jiang.(2024).A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(8),4116-4126. |
| MLA | Gu, Xiaoqian,et al."A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.8(2024):4116-4126. |
入库方式： OAI收割
来源：海洋研究所
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