Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn (II) and the role of H2S in Mn(II) resistance
文献类型：期刊论文
| 作者 | Xu, Xiuli3; Song, Fuhang1; Zhang, Guoliang2; Ma, Linlin4; Yang, Na5,6 |
| 刊名 | ENVIRONMENTAL RESEARCH
 |
| 出版日期 | 2024-04-01 |
| 卷号 | 246页码:10 |
| ISSN号 | 0013-9351 |
| 关键词 | Halomonas sp. MNB13 Proteomic analysis Mn(II) resistance Cysteine metabolism H2S Reactive oxygen species |
| DOI | 10.1016/j.envres.2024.118157 |
| 通讯作者 | Yang, Na(yangna@qdio.ac.cn) |
| 英文摘要 | Halomonas spp. are moderately halophilic bacteria with the ability to tolerate various heavy metals. However, the role of basic cellular metabolism, particularly amino acid metabolism, has not been investigated in Halomonas spp. under excess Mn(II). The strain Halomonas sp. MNB13 was isolated from a deep-sea ferromanganese nodule and can tolerate 80 mM Mn(II). To comprehensively explore the mechanisms underlying its resistance to excess Mn(II), we conducted a comparative proteome analysis. The data revealed that both 10 mM and 50 mM Mn(II) significantly up-regulated the expression of proteins involved in Mn(II) transport (MntE), oxidative stress response (alkyl hydroperoxide reductase and the Suf system), and amino acid metabolism (arginine, cysteine, methionine, and phenylalanine). We further investigated the role of cysteine metabolism in Mn(II) resistance by examining the function of its downstream product, H2S. Consistent with the up-regulation of cysteine desulfurase, we detected an elevated level of H2S in Halomonas sp. MNB13 cells under Mn(II) stress, along with increased intracellular levels of H2O2 and O-2(center dot-). Upon exogenous addition of H2S, we observed a significant restoration of the growth of Halomonas sp. MNB13. Moreover, we identified decreased intracellular levels of H2O2 and O-2(center dot-) in MNB13 cells, which coincided with a decreased formation of Mn-oxides during cultivation. In contrast, in cultures containing NaHS, the residual Mn(II) levels were higher than in cultures without NaHS. Therefore, H2S improves Mn(II) tolerance by eliminating intracellular reactive oxygen species rather than decreasing Mn(II) concentration in solution. Our findings indicate that cysteine metabolism, particularly the intermediate H2S, plays a pivotal role in Mn(II) resistance by mitigating the damage caused by reactive oxygen species. These findings provide new insights into the amino acid mechanisms associated with Mn(II) resistance in bacteria. |
| WOS关键词 | PHENYLALANINE AMMONIA-LYASE ; HYDROGEN-SULFIDE ; OXIDATIVE STRESS ; PLANT-RESPONSES ; CYSTEINE CYCLE ; BACTERIUM ; TOLERANCE ; MECHANISM ; TOXICITY ; CADMIUM |
| 资助项目 | Qingdao Natural Science Foundation, China[23-2-1-174-zyyd-jch] ; Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB42000000] ; Research Foundation for Advanced Talents of Beijing Technology and Business University[19008020158] |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:001161956800001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184580]  |
| 专题 | 海洋研究所_深海极端环境与生命过程研究中心 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yang, Na |
| 作者单位 | 1.Beijing Technol & Business Univ, Sch Light Ind, 11 Fucheng Rd, Beijing 100048, Peoples R China 2.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.China Univ Geosci, Sch Ocean Sci, 29 Xueyuan Rd, Beijing 100083, Peoples R China 4.Griffith Univ, Griffith Inst Drug Discovery, Sch Environm & Sci, Brisbane 4111, Australia 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Xiuli,Song, Fuhang,Zhang, Guoliang,et al. Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn (II) and the role of H2S in Mn(II) resistance[J]. ENVIRONMENTAL RESEARCH,2024,246:10. |
| APA | Xu, Xiuli,Song, Fuhang,Zhang, Guoliang,Ma, Linlin,&Yang, Na.(2024).Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn (II) and the role of H2S in Mn(II) resistance.ENVIRONMENTAL RESEARCH,246,10. |
| MLA | Xu, Xiuli,et al."Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn (II) and the role of H2S in Mn(II) resistance".ENVIRONMENTAL RESEARCH 246(2024):10. |
入库方式： OAI收割
来源：海洋研究所
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