DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses
文献类型：期刊论文
| 作者 | Chai, Zhaoyang3,4,5; Liu, Yuyang3,4,5 ; Jia, Siyang1; Li, Fengting3,4,5; Hu, Zhangxi3,4,5; Deng, Yunyan3,4,5; Yue, Caixia2,3; Tang, Ying-Zhong3,4,5
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2024-02-01 |
| 卷号 | 25期号:3页码:17 |
| ISSN号 | 1661-6596 |
| 关键词 | microalgae DNA/RNA stability resting cysts sediments metabarcoding quantitative real-time PCR |
| DOI | 10.3390/ijms25031724 |
| 通讯作者 | Tang, Ying-Zhong(yingzhong.tang@qdio.ac.cn) |
| 英文摘要 | The ever-increasing applications of metabarcoding analyses for environmental samples demand a well-designed assessment of the stability of DNA and RNA contained in cells that are deposited or buried in marine sediments. We thus conducted a qPCR quantification of the DNA and RNA in the vegetative cells of three microalgae entrapped in facsimile marine sediments and found that >90% of DNA and up to 99% of RNA for all microalgal species were degraded within 60 days at 4 degrees C. A further examination of the potential interference of the relic DNA of the vegetative cells with resting cyst detection in sediments was performed via a metabarcoding analysis in artificial marine sediments spiked with the vegetative cells of two Kareniaceae dinoflagellates and the resting cysts of another three dinoflagellates. The results demonstrated a dramatic decrease in the relative abundances of the two Kareniaceae dinoflagellates in 120 days, while those of the three resting cysts increased dramatically. Together, our results suggest that a positive detection of microalgae via metabarcoding analysis in DNA or RNA extracted from marine sediments strongly indicates the presence of intact or viable cysts or spores due to the rapid decay of relic DNA/RNA. This study provides a solid basis for the data interpretation of metabarcoding surveys, particularly in resting cyst detection. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; HARMFUL ALGAL BLOOMS ; 16S RIBOSOMAL-RNA ; REAL-TIME PCR ; PROPIDIUM MONOAZIDE ; BACTERIAL COMMUNITIES ; EXTRACELLULAR DNA ; GONYAULAX-TAMARENSIS ; SP-NOV ; CELLS |
| 资助项目 | Key Research Infrastructures in the CAS Field Stations of the Chinese Academy of Science |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:001161059700001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184438]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Tang, Ying-Zhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Yellow Sea & East Sea Buoy Observat Stn, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Laoshan Lab, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chai, Zhaoyang,Liu, Yuyang,Jia, Siyang,et al. DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(3):17. |
| APA | Chai, Zhaoyang.,Liu, Yuyang.,Jia, Siyang.,Li, Fengting.,Hu, Zhangxi.,...&Tang, Ying-Zhong.(2024).DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(3),17. |
| MLA | Chai, Zhaoyang,et al."DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.3(2024):17. |
入库方式： OAI收割
来源：海洋研究所
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