Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer
文献类型：期刊论文
| 作者 | Song, Wen1,2,3,4; Xing, Ronge1,2,3 ; Yang, Haoyue1,2; Gao, Kun1,2; Liu, Song1,2,3; Li, Xin1,2; Yu, Huahua1,2,3; Li, Pengcheng1,2,3
|
| 刊名 | LWT-FOOD SCIENCE AND TECHNOLOGY
 |
| 出版日期 | 2023-10-01 |
| 卷号 | 188页码:13 |
| ISSN号 | 0023-6438 |
| 关键词 | Eumelanin Enzyme -assisted optimization Squid ink Tetramer Antioxidant activity |
| DOI | 10.1016/j.lwt.2023.115464 |
| 通讯作者 | Xing, Ronge(xingronge@qdio.ac.cn) |
| 英文摘要 | The investigation into natural eumelanin found in squid ink is an emerging research topic due to its antioxidative properties. However, the methodologies for acquiring pure eumelanin and establishing the intricate relationship between its structure and antioxidative efficacy remain limited. Novel enzyme extraction technique, termed response surface optimization, was employed to synthesize high-purity eumelanin, and its antioxidant activity was evaluated at free radical scavenging and cellular levels and correlated with detailed tetrameric structure prediction for the first time. Results demonstrated that enzymatic extraction technique yielded eumelanin (ANF) nanostructure with stability, boasting particle size of 112.35 nm, a maximum absorption wavelength of 224 nm, a Delta Hpp value of 0.4802 mT, and a purity of 92%. Compared with melanin standard (MelS), ANF had superior free radical scavenging activity, eliminating 89.62% of DPPH radicals, 96.79% of superoxide ion radicals, and 93.20% of hydroxyl radicals, and better repair of cellular oxidative damage. Based on molecular energy, molecular electrostatic potential (MEP), and molecular orbitals by Gaussian calculations, the tetrameric structure (DHICA3-DHI1"' and "DHICA3-DHI1") with significant antioxidant activity contained inside eumelanin was anticipated. Our findings reveal its potential as a natural antioxidant, offering broad prospects for antioxidant food additives or therapeutic applications in nutritional and pharmaceutical ingredients. |
| WOS关键词 | MELANIN ; PROTEINS ; BEHAVIOR ; PIGMENT ; VIVO |
| 资助项目 | Shandong Key R D Plan ; Major Scientific and Technological Innovation Project[2022CXGC020413] ; Fujian Science and Technology Planning Project -STS Program[2021T3013] |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:001109520500001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/183954]  |
| 专题 | 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Xing, Ronge |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 7 Nanhai Rd, Qingdao 266000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Wen,Xing, Ronge,Yang, Haoyue,et al. Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer[J]. LWT-FOOD SCIENCE AND TECHNOLOGY,2023,188:13. |
| APA | Song, Wen.,Xing, Ronge.,Yang, Haoyue.,Gao, Kun.,Liu, Song.,...&Li, Pengcheng.(2023).Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer.LWT-FOOD SCIENCE AND TECHNOLOGY,188,13. |
| MLA | Song, Wen,et al."Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer".LWT-FOOD SCIENCE AND TECHNOLOGY 188(2023):13. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。