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Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus

文献类型：期刊论文

作者Wang, Tingting5,6,7; Wu, Xia3,4; Zhao, Xiaoli5,6; Li, Jiaqi2; Yu, Jian3,4; Sheng, Maozheng3,4; Gao, Mingyuan3,4; Cao, Yutang2; Wang, Jiawen1; Guo, Xiaozhen2
刊名JOURNAL OF INFLAMMATION RESEARCH
出版日期2024
卷号17页码:1241-1253
关键词sevoflurane hippocampus neuroinflammation RNA-seq metabolomics isatin
DOI10.2147/JIR.S448959
通讯作者Guo, Xiaozhen(guoxz@simm.ac.cn) ; Zeng, Kai(fymzk6822@163.com)
英文摘要Purpose: Postoperative cognitive dysfunction (POCD) is a central nervous system complication that occurs after anesthesia, particularly among the elderly. However, the neurological pathogenesis of postoperative cognitive dysfunction remains unclear. The aim of this study was to evaluate the effects of sevoflurane exposure on serum metabolites and hippocampal gene expression in elderly patients and aging mice by metabolomics and transcriptomic analysis and to explore the pathogenesis of sevoflurane induced POCD. Patients and Methods: Human serum samples from five patients over 60 years old were collected before sevoflurane anesthesia and 1 hour after anesthesia. Besides, mice aged at 12 months (n=6 per group) were anesthetized with sevoflurane for 2 hours or with sham procedure. Subsequently, serum and hippocampal tissues were harvested for analysis. Further investigation into the relationship between isatin and neuroinflammation was conducted using BV2 microglial cells. Results: Sevoflurane anesthesia led to the activation of inflammatory pathways, an increased presence of hippocampal astrocytes and microglia, and elevated expression of neuroinflammatory cytokines. Comparative analysis identified 12 differential metabolites that exhibited changes in both human and mouse serum post-sevoflurane anesthesia. Notably, isatin levels were significantly decreased after anesthesia. Notably, isatin levels significantly decreased after anesthesia, a factor known to stimulate proliferation and proinflammatory gene expression in microglia-the pivotal cell type in inflammatory responses. Conclusion: Sevoflurane-induced alterations in serum metabolites in both elderly patients and aging mice, subsequently contributing to increased inflammation in the hippocampus.
WOS关键词EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS ; 3-O-METHYLDOPA ; ISATIN
资助项目National Natural Science Foundation of China[82104253] ; National Natural Science Foundation of China[82300979] ; Jointed PI Program from Shanghai Changning Maternity and Infant Health Hospital[11300-412311-22043] ; Changning Maternity and Infant Health Hospital Scientific Research Staring Foundation[2020Y-15] ; Shanghai Changning District Health Commission Youth Program[2022QN14]
WOS研究方向Immunology
语种英语
出版者DOVE MEDICAL PRESS LTD
WOS记录号WOS:001170492100001
源URL[http://119.78.100.183/handle/2S10ELR8/309874]  
专题中国科学院上海药物研究所
通讯作者Guo, Xiaozhen; Zeng, Kai
作者单位1.Wuhan Univ, Coll Life Sci, Wuhan, Peoples R China
2.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai, Peoples R China
3.East China Normal Univ, Sch Life Sci, Shanghai, Peoples R China
4.East China Normal Univ, Inst Biomed Sci, Shanghai Key Lab Regulatory Biol, Shanghai, Peoples R China
5.Changning Matern & Infant Hlth Hosp, Dept Anesthesiol, Shanghai, Peoples R China
6.Shanghai Univ Tradit Chinese Med, Peoples Hosp 7, Shanghai, Peoples R China
7.Fujian Med Univ, Affiliated Hosp 1, Anesthesiol Res Inst, Dept Anesthesiol, Fuzhou, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Tingting,Wu, Xia,Zhao, Xiaoli,et al. Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus[J]. JOURNAL OF INFLAMMATION RESEARCH,2024,17:1241-1253.
APA Wang, Tingting.,Wu, Xia.,Zhao, Xiaoli.,Li, Jiaqi.,Yu, Jian.,...&Zeng, Kai.(2024).Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus.JOURNAL OF INFLAMMATION RESEARCH,17,1241-1253.
MLA Wang, Tingting,et al."Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus".JOURNAL OF INFLAMMATION RESEARCH 17(2024):1241-1253.

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来源：上海药物研究所

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