Eliminating drug target interference with specific antibody or its F(ab′)2 fragment in the bridging immunogenicity assay
文献类型：期刊论文
| 作者 | Deng, Xiaojie4; Hou, Yingying3,4; Yuan, Wenyi4; Yang, Hongzhou1; Guo, Ruowen1; Liu, Tingting3,4; Liu, Yongzhen4 ; Xu, Junjiu4; Liu, Heng1; Gong, Likun2,3,4
|
| 刊名 | BIOANALYSIS
 |
| 出版日期 | 2024-02-22 |
| 页码 | 14 |
| ISSN号 | 1757-6180 |
| 关键词 | anti-drug antibody IgE immunogenicity mitigation target interference |
| DOI | 10.4155/bio-2023-0191 |
| 通讯作者 | Qin, Qiuping(qpqin@cdser.simm.ac.cn) |
| 英文摘要 | Background: DB-1003 is a humanized anti-IgE monoclonal antibody with higher affinity than omalizumab. In the affinity capture elution (ACE)-based bridging electrochemiluminescent immunoassay (ECLIA) for antibodies to DB-1003, monkey serum IgE caused false-positive results. Materials & methods: The target-specific antibody or its F(ab ')(2) fragment was used to mitigate drug target interference in an ACE-based bridging ECLIA for the detection of anti-DB-1003 antibodies. Results: The sensitivity of the developed assay was at least 100 ng/ml. When the ADA concentration was 250 ng/ml, the assay tolerated at least 20.0 mu g/ml of the monkey IgE. Conclusion: Incorporating the target-specific antibody or its F(ab ')(2) fragment can overcome the interference from monkey serum IgE in ACE-based bridging ECLIA for anti-DB-1003 antibody detection. |
| WOS关键词 | THERAPEUTIC PROTEINS ; HOST ANTIBODIES ; RECOMMENDATIONS ; EXTRACTION ; ADA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | TAYLOR & FRANCIS LTD |
| WOS记录号 | WOS:001168656400001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309916]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Qin, Qiuping |
| 作者单位 | 1.Longbio Pharm Suzhou Co Ltd, Suzhou 215558, Peoples R China 2.Univ Chinese Acad Sci, Sch Pharm, Beijing 100049, Peoples R China 3.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Xiaojie,Hou, Yingying,Yuan, Wenyi,et al. Eliminating drug target interference with specific antibody or its F(ab′)2 fragment in the bridging immunogenicity assay[J]. BIOANALYSIS,2024:14. |
| APA | Deng, Xiaojie.,Hou, Yingying.,Yuan, Wenyi.,Yang, Hongzhou.,Guo, Ruowen.,...&Qin, Qiuping.(2024).Eliminating drug target interference with specific antibody or its F(ab′)2 fragment in the bridging immunogenicity assay.BIOANALYSIS,14. |
| MLA | Deng, Xiaojie,et al."Eliminating drug target interference with specific antibody or its F(ab′)2 fragment in the bridging immunogenicity assay".BIOANALYSIS (2024):14. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。