The cytosolic aminotransferase VAS1 coordinates aromatic amino acid biosynthesis and metabolism
文献类型：期刊论文
| 作者 | Wu, Jie9; Chen, Yanhong8; Huang, Yuxin7,9; Hao, Bingbing6; Dai, Shengkun5; Zhao, Lingling9; Zhao, Zhehui4; Zhao, Cuihuan8; Zhang, Limin3; Li, Yunhai3 |
| 刊名 | SCIENCE ADVANCES
 |
| 出版日期 | 2024-01-12 |
| 卷号 | 10期号:2页码:11 |
| ISSN号 | 2375-2548 |
| DOI | 10.1126/sciadv.adk0738 |
| 通讯作者 | Zhu, Wentao(qiao.zhao@siat.ac.cn) ; Zhao, Qiao(wentaozhu@cau.edu.cn) |
| 英文摘要 | The aromatic amino acids (AAAs) phenylalanine, tyrosine, and tryptophan are basic protein units and precursors of diverse specialized metabolites that are essential for plant growth. Despite their significance, the mechanisms that regulate AAA homeostasis remain elusive. Here, we identified a cytosolic aromatic aminotransferase, REVERSAL OF SAV3 PHENOTYPE 1 (VAS1), as a suppressor of arogenate dehydrogenase 2 (adh2) in Arabidopsis (Arabidopsis thaliana). Genetic and biochemical analyses determined that VAS1 uses AAAs as amino donors, leading to the formation of 3-carboxyphenylalanine and 3-carboxytyrosine. These pathways represent distinct routes for AAA metabolism that are unique to specific plant species. Furthermore, we show that VAS1 is responsible for cytosolic AAA biosynthesis, and its enzymatic activity can be inhibited by 3-carboxyphenylalanine. These findings provide valuable insights into the crucial role of VAS1 in producing 3-carboxy AAAs, notably via recycling of AAAs in the cytosol, which maintains AAA homeostasis and allows plants to effectively coordinate the complex metabolic and biosynthetic pathways of AAAs. |
| WOS关键词 | ARABIDOPSIS-THALIANA ; PHENYLALANINE BIOSYNTHESIS ; ANTHRANILATE SYNTHASE ; SHIKIMATE PATHWAY ; SALICYLIC-ACID ; TYROSINE ; TRANSFORMATION ; 3-(3-CARBOXYPHENYL)ALANINE ; ISOCHORISMATE ; EVOLUTION |
| 资助项目 | National Key Research and Development Program of China[2019YFA0903900] ; National Natural Science Foundation of China[32300233] ; Guangdong Provincial Key laboratory of Synthetic Genomics[2023B1212060054] ; Shenzhen Key laboratory of Synthetic Genomics[ZdSYS201802061806209] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
| WOS记录号 | WOS:001139921200012 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309919]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhu, Wentao; Zhao, Qiao |
| 作者单位 | 1.China Agr Univ, Coll Sci, Innovat Ctr Pesticide Res, Dept Appl Chem, Beijing 100193, Peoples R China 2.Southern Univ Sci & Technol, Key Lab Mol Design Plant Cell Factory Guangdong Hi, SUStech PKU Inst Plant & Food Sci, Sch Life Sci,Dept Biol, Shenzhen 518055, Peoples R China 3.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Biol, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol,Innovat Acad Seed Design, Beijing 100101, Peoples R China 4.Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Bioact Subst & Funct Nat Med, Beijing Key Lab Act Subst Discovery & Druggabil Ev, Inst Mat Med,Dept Med Chem, Beijing 100050, Peoples R China 5.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, Shenzhen 518055, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Tsinghua Univ, Ctr Plant Biol, Sch Life Sci, Beijing 100084, Peoples R China 9.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen Key Lab Synthet Genom,Guangdong Prov Key, Shenzhen 518055, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Jie,Chen, Yanhong,Huang, Yuxin,et al. The cytosolic aminotransferase VAS1 coordinates aromatic amino acid biosynthesis and metabolism[J]. SCIENCE ADVANCES,2024,10(2):11. |
| APA | Wu, Jie.,Chen, Yanhong.,Huang, Yuxin.,Hao, Bingbing.,Dai, Shengkun.,...&Zhao, Qiao.(2024).The cytosolic aminotransferase VAS1 coordinates aromatic amino acid biosynthesis and metabolism.SCIENCE ADVANCES,10(2),11. |
| MLA | Wu, Jie,et al."The cytosolic aminotransferase VAS1 coordinates aromatic amino acid biosynthesis and metabolism".SCIENCE ADVANCES 10.2(2024):11. |
入库方式： OAI收割
来源：上海药物研究所
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