Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease
文献类型：期刊论文
| 作者 | Qiao, Huarui8; Li, Lingyun6,7; Wang, Lu7; Yu, Haijun4,5 ; Hu, Fangzheng8; Zhou, Xin8; Yang, Hongzhao8; Xu, Jianfeng8; Meng, Xiangjing1; Geng, Yong6,7
|
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2023-07-01 |
| 卷号 | 207页码:106267 |
| 关键词 | SARS-CoV-2 Papain like protease (PLpro) Nanobody PLpro inhibitor |
| DOI | 10.1016/j.pep.2023.106267 |
| 文献子类 | Article |
| 英文摘要 | Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity experiments demonstrated that two nanobodies had a significant inhibitory effect on the PLpro. These nanobodies should therefore be investigated as candidates for the treatment of coronaviruses. |
| WOS关键词 | REPLICATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000980997900001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309648]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xu, Jianfeng; Meng, Xiangjing; Geng, Yong; Dai, Yuanyuan |
| 作者单位 | 1.Shandong Acad Pharmaceut Sci, Shandong Prov Key Lab Biopharmaceut, Jinan 250101, Peoples R China 2.Chinese Acad Med Sci, Hebei Canc Hosp, Natl Canc Ctr, Natl Clin Res Ctr Canc, Langfang 065001, Peoples R China; 3.Chinese Acad Med Sci & Peking Union Med Coll, Canc Hosp, Natl Clin Res Ctr Canc, Natl Canc Ctr,Dept Pharm, Beijing 100021, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 7.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 8.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Biopharmaceut, Shanghai 201306, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Qiao, Huarui,Li, Lingyun,Wang, Lu,et al. Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease[J]. PROTEIN EXPRESSION AND PURIFICATION,2023,207:106267. |
| APA | Qiao, Huarui.,Li, Lingyun.,Wang, Lu.,Yu, Haijun.,Hu, Fangzheng.,...&Dai, Yuanyuan.(2023).Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease.PROTEIN EXPRESSION AND PURIFICATION,207,106267. |
| MLA | Qiao, Huarui,et al."Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease".PROTEIN EXPRESSION AND PURIFICATION 207(2023):106267. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。