A procedure for producing an anti-AXL nanobody in E. coli
文献类型：期刊论文
| 作者 | Lan, Zhongyun6,7,8; Li, Lingyun5,6; Sun, Yili4; Meng, Xiangjing3; Shi, Zhenzhong6; Du, Mengyang6,8; Wang, Hui6,8; Sun, Zengchao6,8; Cui, Qianqian6; Wang, Lu6 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2023-07-01 |
| 卷号 | 207页码:106268 |
| 关键词 | AXL Nanobody Expression Purification |
| DOI | 10.1016/j.pep.2023.106268 |
| 文献子类 | Article |
| 英文摘要 | As one of the receptors of the TAM family, AXL plays a vital role in stem cell maintenance, angiogenesis, immune escape of viruses and drug resistance against tumors. In this study, the truncated extracellular segment con-taining two immunoglobulin-like domains of human AXL (AXL-IG), which has been confirmed to bind growth arrest specific 6 (GAS6) by structural studies [1], was expressed in a prokaryotic expression system and then purified. Immunizing camelid with the purified AXL-IG as antigen could lead to the production of unique nanobodies composed of only variable domain of heavy chain of heavy-chain antibody (VHH), which are around 15 kD and stable. We screened out a nanobody A-LY01 specific binding to AXL-IG. We further determined the affinity of A-LY01 to AXL-IG and revealed that A-LY01 could specifically recognize full-length AXL on the surface of HEK 293T/17 cells. Our study provides appropriate support for the development of diagnostic reagents and antibody therapeutics targeting AXL. |
| WOS关键词 | TAM RECEPTORS ; TYROSINE ; RESISTANCE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000981418400001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309650]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Geng, Yong; Zhu, Yongheng; Dai, Yuanyuan |
| 作者单位 | 1.Chinese Acad Med Sci, Natl Canc Ctr, Natl Clin Res Ctr Canc, Hebei Canc Hosp, Langfang 065001, Peoples R China 2.Chinese Acad Med Sci & Peking Union Med Coll, Dept Pharm, Natl Canc Ctr, Natl Clin Res Ctr Canc,Canc Hosp, Beijing 100021, Peoples R China; 3.Shandong Acad Pharmaceut Sci, Shandong Prov Key Lab Biopharmaceut, Jinan 250101, Peoples R China; 4.Bohai Rim Adv Res Inst Drug Discovery, Drug Discovery Shandong Lab, Yantai 264117, Shandong, Peoples R China; 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 7.Minist Agr, Lab Qual & Safety Risk Assessment Aquat Prod Stora, Shanghai 201306, Peoples R China; 8.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lan, Zhongyun,Li, Lingyun,Sun, Yili,et al. A procedure for producing an anti-AXL nanobody in E. coli[J]. PROTEIN EXPRESSION AND PURIFICATION,2023,207:106268. |
| APA | Lan, Zhongyun.,Li, Lingyun.,Sun, Yili.,Meng, Xiangjing.,Shi, Zhenzhong.,...&Dai, Yuanyuan.(2023).A procedure for producing an anti-AXL nanobody in E. coli.PROTEIN EXPRESSION AND PURIFICATION,207,106268. |
| MLA | Lan, Zhongyun,et al."A procedure for producing an anti-AXL nanobody in E. coli".PROTEIN EXPRESSION AND PURIFICATION 207(2023):106268. |
入库方式： OAI收割
来源：上海药物研究所
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