Identification of an effective fraction from Ampelopsis Radix with anti-dengue virus activities in vitro and in vivo
文献类型：期刊论文
| 作者 | Tian, Chunyang5; Huang, Hefei5; Zheng, Yuanru5; He, Xuemei5; Yan, Lijun5; Shi, Lingzhu5; Yang, Tangjia5; Chen, Xi5; Yang, Jiabin5; Lu, Zibin5 |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2023-06-12 |
| 卷号 | 309页码:116339 |
| 关键词 | Ampelopsis Radix Dengue virus Antiviral ICR suckling Mice AG129 mice |
| DOI | 10.1016/j.jep.2023.116339 |
| 文献子类 | Article |
| 英文摘要 | Ethnopharmacological relevance: Dengue virus (DENV) infection is a global public health issue without effective therapeutic interventions. Chinese medicine with heat-clearing and detoxifying properties has been frequently used in the treatment of viral infection. Ampelopsis Radix (AR) is a traditional Chinese medicine for clearing heat and detoxification that has been widely used in the prevention and treatment of infectious diseases. However, no studies on the effects of AR against viral infection have been reported, thus far. Aim of the study: To explore the anti-DENV activities of the fraction (AR-1) obtained from AR both in vitro and in vivo. Materials and methods: The chemical composition of AR-1 was identified by liquid chromatography-tandem MS (LC-MS/MS). The antiviral activities of AR-1 were studied in baby hamster kidney fibroblast BHK-21 cells, ICR suckling mice and induction of interferon alpha/beta (IFN-alpha/beta) and IFN-gamma R / (AG129) mice. Results: Based on LC-MS/MS analysis, 60 compounds (including flavonoids, phenols, anthraquinones, alkaloids and other types) were tentatively characterized from AR-1. AR-1 inhibited the cytopathic effect, the production of progeny virus and the synthesis of viral RNA and proteins by blocking DENV-2 binding to BHK-21 cells. Moreover, AR-1 significantly attenuated weight loss, decreased clinical scores and prolonged the survival of DENV-infected ICR suckling mice. Critically, the viral load in blood, brain and kidney tissues and the patho-logical changes in brain were remarkably alleviated after AR-1 treatment. Further study on AG129 mice showed that AR-1 obviously improved the clinical manifestations and survival rate, reduced viremia, attenuated gastric distension and relieved the pathological lesions caused by DENV. Conclusions: In summary, this is the first report that AR-1 exhibits anti-DENV effects both in vitro and in vivo, which suggests that AR-1 may be developed as a therapeutic candidate against DENV infection. |
| WOS关键词 | DOMAIN-III ; GALLIC ACID ; GLYCOPROTEIN ; PROTEIN ; VECTOR ; ROOT |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| 出版者 | ELSEVIER IRELAND LTD |
| WOS记录号 | WOS:000956559000001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309669]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Liu, Junshan; Yu, Linzhong |
| 作者单位 | 1.Southern Med Univ, Zhujiang Hosp, Dept Pharm, Guangzhou 510280, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med SIMM, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Southern Med Univ, Guangdong Prov Key Lab Chinese Med Pharmaceut, Guangzhou 510515, Peoples R China; 4.Southern Med Univ, Sch Publ Hlth, Guangdong Prov Key Lab Trop Dis Res, Guangzhou 510515, Peoples R China; 5.Southern Med Univ, Sch Tradit Chinese Med, Level Res Lab 3, Guangdong Prov Key Lab Chinese Med Pharmaceut,Stat, Guangzhou 510515, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Tian, Chunyang,Huang, Hefei,Zheng, Yuanru,et al. Identification of an effective fraction from Ampelopsis Radix with anti-dengue virus activities in vitro and in vivo[J]. JOURNAL OF ETHNOPHARMACOLOGY,2023,309:116339. |
| APA | Tian, Chunyang.,Huang, Hefei.,Zheng, Yuanru.,He, Xuemei.,Yan, Lijun.,...&Yu, Linzhong.(2023).Identification of an effective fraction from Ampelopsis Radix with anti-dengue virus activities in vitro and in vivo.JOURNAL OF ETHNOPHARMACOLOGY,309,116339. |
| MLA | Tian, Chunyang,et al."Identification of an effective fraction from Ampelopsis Radix with anti-dengue virus activities in vitro and in vivo".JOURNAL OF ETHNOPHARMACOLOGY 309(2023):116339. |
入库方式： OAI收割
来源：上海药物研究所
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