Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds
文献类型：期刊论文
| 作者 | Lin, Jia-Ling8,9; Fang, Xin7; Li, Jian-Xu9; Chen, Zhi-Wen6; Wu, Wen-Kai9; Guo, Xiao-Xiang9; Liu, Ning-Jing5; Huang, Jia-Fa9; Chen, Fang-Yan9; Wang, Ling-Jian9 |
| 刊名 | NATURE PLANTS
 |
| 出版日期 | 2023-04-01 |
| 卷号 | 9期号:4页码:605-+ |
| DOI | 10.1038/s41477-023-01376-2 |
| 文献子类 | Article |
| 英文摘要 | This study identified two dirigent proteins responsible for the biosynthesis of the two enantiomers of gossypol in cotton. Editing one of the dirigent proteins selectively removed the toxic (-)-gossypol in cotton seeds without affecting pest resistance. Axial chirality of biaryls can generate varied bioactivities. Gossypol is a binaphthyl compound made by cotton plants. Of its two axially chiral isomers, (-)-gossypol is the bioactive form in mammals and has antispermatogenic activity, and its accumulation in cotton seeds poses health concerns. Here we identified two extracellular dirigent proteins (DIRs) from Gossypium hirsutum, GhDIR5 and GhDIR6, which impart the hemigossypol oxidative coupling into (-)- and (+)-gossypol, respectively. To reduce cotton seed toxicity, we disrupted GhDIR5 by genome editing, which eliminated (-)-gossypol but had no effects on other phytoalexins, including (+)-gossypol, that provide pest resistance. Reciprocal mutagenesis identified three residues responsible for enantioselectivity. The (-)-gossypol-forming DIRs emerged later than their enantiocomplementary counterparts, from tandem gene duplications that occurred shortly after the cotton genus diverged. Our study offers insight into how plants control enantiomeric ratios and how to selectively modify the chemical spectra of cotton plants and thereby improve crop quality. |
| WOS关键词 | DOMAIN-CONTAINING PROTEIN ; BIOSYNTHESIS ; (-)-GOSSYPOL ; SYNTHASE ; (+)-GOSSYPOL ; METABOLISM ; EXPRESSION ; REDUCTION |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000950522700004 |
| 出版者 | NATURE PORTFOLIO |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309671]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Chen, Xiao-Ya; Huang, Jin-Quan |
| 作者单位 | 1.Shanghai Chenshan Bot Garden, Shanghai Key Lab Plant Funct Genom & Resources, Shanghai, Peoples R China 2.John Innes Ctr, Norwich, Norfolk, England; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 4.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai, Peoples R China; 5.East China Normal Univ, Sch Life Sci, Shanghai, Peoples R China; 6.Hainan Yazhou Bay Seed Lab, Sanya, Peoples R China; 7.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochemistry & Plant Resources Wes, Kunming, Peoples R China; 8.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 9.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci,Natl Key Lab Plan, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lin, Jia-Ling,Fang, Xin,Li, Jian-Xu,et al. Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds[J]. NATURE PLANTS,2023,9(4):605-+. |
| APA | Lin, Jia-Ling.,Fang, Xin.,Li, Jian-Xu.,Chen, Zhi-Wen.,Wu, Wen-Kai.,...&Huang, Jin-Quan.(2023).Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds.NATURE PLANTS,9(4),605-+. |
| MLA | Lin, Jia-Ling,et al."Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds".NATURE PLANTS 9.4(2023):605-+. |
入库方式： OAI收割
来源：上海药物研究所
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