First trans-eunicellane terpene synthase in bacteria
文献类型：期刊论文
| 作者 | Li, Zining7; Xu, Baofu2,3,7; Kojasoy, Volga6; Ortega, Teresa6; Adpressa, Donovon A.5; Ning, Wenbo7; Wei, Xiuting7; Liu, Jamin1,7; Tantillo, Dean J.6; Loesgen, Sandra4,7 |
| 刊名 | CHEM
 |
| 出版日期 | 2023-03-09 |
| 卷号 | 9期号:3页码:698-708 |
| DOI | 10.1016/j.chempr.2022.12.006 |
| 文献子类 | Article |
| 英文摘要 | Terpenoids are the largest family of natural products, but prokary-otes are vastly underrepresented in this chemical space. However, genomics supports vast untapped biosynthetic potential for terpe-noids in bacteria. We discovered the first trans-eunicellane terpene synthase (TS), AlbS from Streptomyces albireticuli NRRL B-1670, in nature. Mutagenesis, deuterium labeling studies, and quantum chemical calculations provided extensive support for its cyclization mechanism. In addition, parallel stereospecific labeling studies with Bnd4, a cis-eunicellane TS, revealed a key mechanistic distinc-tion between these two enzymes. AlbS highlights bacteria as a valu-able source of novel terpenoids, expands our understanding of the eunicellane family of natural products and the enzymes that bio-synthesize them, and provides a model system to address funda-mental questions about the chemistry of 6,10-bicyclic ring systems. |
| WOS关键词 | ABSOLUTE-CONFIGURATION ; ELEUTHEROBIN ; DITERPENOIDS ; ENZYMOLOGY ; PRODUCT |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000972568300001 |
| 出版者 | CELL PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309675]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Rudolf, Jeffrey D. |
| 作者单位 | 1.NYU, Tandon Sch Engn, Brooklyn, NY USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 3.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai, Shandong, Peoples R China; 4.Univ Florida, Whitney Lab Marine Biosci, St Augustine, FL USA; 5.Loxo Oncol Inc, Louisville, CO USA; 6.Univ Calif Davis, Dept Chem, Davis, CA USA; 7.Univ Florida, Dept Chem, Gainesville, FL 32611 USA; |
| 推荐引用方式 GB/T 7714 | Li, Zining,Xu, Baofu,Kojasoy, Volga,et al. First trans-eunicellane terpene synthase in bacteria[J]. CHEM,2023,9(3):698-708. |
| APA | Li, Zining.,Xu, Baofu.,Kojasoy, Volga.,Ortega, Teresa.,Adpressa, Donovon A..,...&Rudolf, Jeffrey D..(2023).First trans-eunicellane terpene synthase in bacteria.CHEM,9(3),698-708. |
| MLA | Li, Zining,et al."First trans-eunicellane terpene synthase in bacteria".CHEM 9.3(2023):698-708. |
入库方式： OAI收割
来源：上海药物研究所
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