Development of a simultaneous quantification method for the gut microbiota-derived core nutrient metabolome in mice and its application in studying host-microbiota interaction
文献类型：期刊论文
| 作者 | Xu, Hualing4,5; Wang, Jiawen2,3; Liu, Yameng4; Wang, Yangyang1,5; Zhong, Xianchun4; Li, Cuina4; Wang, Kanglong4; Guo, Xiaozhen4; Xie, Cen4,5
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| 刊名 | ANALYTICA CHIMICA ACTA
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| 出版日期 | 2023-04-22 |
| 卷号 | 1251页码:341039 |
| 关键词 | Obesity Gut microbiota Nutrient metabolome LC-MS Amino acid |
| DOI | 10.1016/j.aca.2023.341039 |
| 文献子类 | Article |
| 英文摘要 | The gut microbiota interacts with the host via production of various metabolites of dietary nutrients. Herein, we proposed the concept of the gut microbiota-derived core nutrient metabolome, which covers 43 metabolites in carbohydrate metabolism, glycolysis, tricarboxylic acid cycle and amino acid metabolism, and established a quantitative UPLC-Q/TOF-MS method through 3-nitrophenylhydrazine derivatization to investigate the influ-ence of obesity on the gut microbiota in mice. All metabolites could be simultaneously analyzed via separation on a BEH C18 column within 18 min. The lower limits of quantification of most analytes were less than 1 mu M. Validation results demonstrated suitability for the analysis of mouse fecal samples. The method was then applied to detect the gut microbiota-derived nutrient metabolome in the feces of high-fat diet induced obese (DIO) and ob/ob (leptin-deficient) mice, as well as obesity-prone (OP) and obesity-resistant (OR) mice. Compared to the control groups, there were 13, 23 and 10 differentially abundant metabolites detected in ob/ob, DIO and OP groups, respectively. Among them, amino acids including leucine, isoleucine, glycine, methionine, tyrosine and glutamine were co-downregulated in the obese or OP mice and exhibited inverse association with body weight. 16S rDNA analysis revealed that the genera Lactobacillus and Dubosiella were also inversely associated with body weight and positively correlated with fecal amino acids. Collectively, our work provides an effective and simplified method for simultaneous quantifying the gut microbiota-derived core nutrient metabolome in mouse feces, which could assist various future studies on host-microbiota metabolic interaction. |
| WOS关键词 | CHAIN FATTY-ACIDS ; OBESITY ; DISEASE ; HEALTH |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000951169500001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309679]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Guo, Xiaozhen; Xie, Cen |
| 作者单位 | 1.Shanghai Inst Pharmaceut Ind, China State Inst Pharmaceut Ind, State Key Lab New Drug & Pharmaceut Proc, Shanghai 201203, Peoples R China 2.East China Normal Univ, Sch Life Sci, Shanghai 2022241, Peoples R China; 3.East China Normal Univ, Inst Biomed Sci, Shanghai Key Lab Regulatory Biol, Shanghai 2022241, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 5.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xu, Hualing,Wang, Jiawen,Liu, Yameng,et al. Development of a simultaneous quantification method for the gut microbiota-derived core nutrient metabolome in mice and its application in studying host-microbiota interaction[J]. ANALYTICA CHIMICA ACTA,2023,1251:341039. |
| APA | Xu, Hualing.,Wang, Jiawen.,Liu, Yameng.,Wang, Yangyang.,Zhong, Xianchun.,...&Xie, Cen.(2023).Development of a simultaneous quantification method for the gut microbiota-derived core nutrient metabolome in mice and its application in studying host-microbiota interaction.ANALYTICA CHIMICA ACTA,1251,341039. |
| MLA | Xu, Hualing,et al."Development of a simultaneous quantification method for the gut microbiota-derived core nutrient metabolome in mice and its application in studying host-microbiota interaction".ANALYTICA CHIMICA ACTA 1251(2023):341039. |
入库方式： OAI收割
来源：上海药物研究所
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