Sensitivity-enhanced nanoplasmonic biosensor using direct immobilization of two engineered nanobodies for SARS-CoV-2 spike receptor-binding domain detection
文献类型：期刊论文
| 作者 | Ma, Zhengtai3,4; Sun, Zengchao2; Lv, Xiaoqing4; Chen, Hongda4; Geng, Yong2 ; Geng, Zhaoxin1
|
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2023-05-15 |
| 卷号 | 383页码:133575 |
| 关键词 | Nanoplasmonics Biosensor COVID-19 Nanobody Immobilization High -throughput |
| DOI | 10.1016/j.snb.2023.133575 |
| 文献子类 | Article |
| 英文摘要 | Sensitive, rapid, and easy-to-implement biosensors are critical in responding to highly contagious and fast -spreading severe acute respiratory syndrome coronavirus (SARS-CoV-2) mutations, enabling early infection screening for appropriate isolation and treatment measures to prevent the spread of the virus. Based on the sensing principle of localized surface plasmon resonance (LSPR) and nanobody immunological techniques, an enhanced sensitivity nanoplasmonic biosensor was developed to quantify the SARS-CoV-2 spike receptor-binding domain (RBD) in serum within 30 min. The lowest concentration in the linear range can be detected down to 0.01 ng/mL by direct immobilization of two engineered nanobodies. Both the sensor fabrication process and immune strategy are facile and inexpensive, with the potential for large-scale application. The designed nano-plasmonic biosensor achieved excellent specificity and sensitivity for SARS-CoV-2 spike RBD, providing a po-tential option for accurate early screening of the novel coronavirus disease 2019 (COVID-19). |
| WOS关键词 | NANOPARTICLE ; EQUILIBRIUM |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| WOS记录号 | WOS:000979647900001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309685]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Geng, Yong; Geng, Zhaoxin |
| 作者单位 | 1.Minzu Univ China, Sch Informat Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, State Key Lab Drug Res, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing, Peoples R China; 4.Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ma, Zhengtai,Sun, Zengchao,Lv, Xiaoqing,et al. Sensitivity-enhanced nanoplasmonic biosensor using direct immobilization of two engineered nanobodies for SARS-CoV-2 spike receptor-binding domain detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,383:133575. |
| APA | Ma, Zhengtai,Sun, Zengchao,Lv, Xiaoqing,Chen, Hongda,Geng, Yong,&Geng, Zhaoxin.(2023).Sensitivity-enhanced nanoplasmonic biosensor using direct immobilization of two engineered nanobodies for SARS-CoV-2 spike receptor-binding domain detection.SENSORS AND ACTUATORS B-CHEMICAL,383,133575. |
| MLA | Ma, Zhengtai,et al."Sensitivity-enhanced nanoplasmonic biosensor using direct immobilization of two engineered nanobodies for SARS-CoV-2 spike receptor-binding domain detection".SENSORS AND ACTUATORS B-CHEMICAL 383(2023):133575. |
入库方式： OAI收割
来源：上海药物研究所
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