Superoxide anion monitoring in epileptic brains with a near-infrared fluorescent probe
文献类型：期刊论文
| 作者 | Ying, Weiwu3; Dong, Fuxing3; Shi, Yifan3; Zhan, Ziyi3; Wang, Shuwan3; Lv, Li3; Liu, Huizhen3; Liu, Ling3; Zheng, Youguang3; Zhang, Ling1,2,3 |
| 刊名 | DYES AND PIGMENTS
 |
| 出版日期 | 2023-05-01 |
| 卷号 | 213页码:111155 |
| 关键词 | Fluorescent probe Near -infrared Superoxide anions Epilepsy |
| DOI | 10.1016/j.dyepig.2023.111155 |
| 文献子类 | Article |
| 英文摘要 | Epilepsy is a chronic neurological disorder, and the pathophysiological progression to status epilepticus is closely associated with oxidative stress. Superoxide anions (O2 center dot-), as the main, most important precursor of other reactive oxygen species (ROS), play a crucial role in seizure-induced brain damage. However, detecting the actual O2 center dot -levels and understanding the physiological roles of O2 center dot in epileptic brains remain challenging due to the lack of effective in vivo detection tools. Herein, a new near-infrared fluorescent probe, MB-SO, was prepared for the determination of endogenous O2 center dot-in brains experiencing pentylenetetrazole (PTZ)-induced epileptic seizures. MB -SO exhibits high sensitivity (detection limit of 14 nM), good selectivity and a fast response towards O2 center dot-. MB-SO was applied in situ to monitor endogenous O2 center dot-in living HT-22 cells and living mice. Using this probe, we visualized and quantitatively detected endogenous O2 center dot-for the first time in the hippocampi of PTZ-induced epileptic mouse brains. MB-SO also displayed a positive correlation between high O2 center dot-levels in epileptic brains and epileptogenesis. These results indicated that the probe MB-SO is an efficient tool for monitoring endogenous O2 center dot-in vivo and exploring the pathogenesis of epilepsy. |
| WOS关键词 | OXIDATIVE STRESS ; REACTIVE OXYGEN ; MITOCHONDRIAL DYSFUNCTION ; ENDOGENOUS SUPEROXIDE ; HYDROGEN-SULFIDE ; NADPH OXIDASE ; LIVING CELLS ; LIVE CELLS ; ACTIVATION ; GENERATION |
| WOS研究方向 | Chemistry ; Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000948916400001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309698]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zheng, Youguang; Zhang, Ling |
| 作者单位 | 1.Nanjing Univ, State Key Lab Coordinat Chem, Nanjing 210023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 3.Xuzhou Med Univ, Publ Expt Res Ctr, Sch Pharm, Jiangsu Key Lab New Drug Res & Clin Pharm, Xuzhou 221002, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ying, Weiwu,Dong, Fuxing,Shi, Yifan,et al. Superoxide anion monitoring in epileptic brains with a near-infrared fluorescent probe[J]. DYES AND PIGMENTS,2023,213:111155. |
| APA | Ying, Weiwu.,Dong, Fuxing.,Shi, Yifan.,Zhan, Ziyi.,Wang, Shuwan.,...&Zhang, Ling.(2023).Superoxide anion monitoring in epileptic brains with a near-infrared fluorescent probe.DYES AND PIGMENTS,213,111155. |
| MLA | Ying, Weiwu,et al."Superoxide anion monitoring in epileptic brains with a near-infrared fluorescent probe".DYES AND PIGMENTS 213(2023):111155. |
入库方式： OAI收割
来源：上海药物研究所
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