Catalytic innovation underlies independent recruitment of polyketide synthases in cocaine and hyoscyamine biosynthesis
文献类型：期刊论文
| 作者 | Tian, Tian; Wang, Yong-Jiang; Huang, Jian-Ping; Li, Jie; Xu, Bingyan; Chen, Yin; Wang, Li ; Yang, Jing; Yan, Yijun; Huang, Sheng-Xiong
|
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2022 |
| 卷号 | 13期号:1页码:4994 |
| 关键词 | TROPANE ALKALOID BIOSYNTHESIS CHAIN-LENGTH CONTROL |
| DOI | 10.1038/s41467-022-32776-1 |
| 英文摘要 | Cocaine is a tropane alkaloid produced by Erythroxylum novogranatense. Here the authors identify two polyketide synthases involved in cocaine biosynthesis and provide insight into the parallel evolution of these enzymes in tropane alkaloids-producing plants. Tropane alkaloids such as hyoscyamine and cocaine are of importance in medicinal uses. Only recently has the hyoscyamine biosynthetic machinery become complete. However, the cocaine biosynthesis pathway remains only partially elucidated. Here we characterize polyketide synthases required for generating 3-oxo-glutaric acid from malonyl-CoA in cocaine biosynthetic route. Structural analysis shows that these two polyketide synthases adopt distinctly different active site architecture to catalyze the same reaction as pyrrolidine ketide synthase in hyoscyamine biosynthesis, revealing an unusual parallel/convergent evolution of biochemical function in homologous enzymes. Further phylogenetic analysis suggests lineage-specific acquisition of polyketide synthases required for tropane alkaloid biosynthesis in Erythroxylaceae and Solanaceae species, respectively. Overall, our work elucidates not only a key unknown step in cocaine biosynthesis pathway but also, more importantly, structural and biochemical basis for independent recruitment of polyketide synthases in tropane alkaloid biosynthesis, thus broadening the understanding of conservation and innovation of biosynthetic catalysts. |
| WOS记录号 | WOS:000845603400016 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74665]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Tian, Tian,Wang, Yong-Jiang,Huang, Jian-Ping,et al. Catalytic innovation underlies independent recruitment of polyketide synthases in cocaine and hyoscyamine biosynthesis[J]. NATURE COMMUNICATIONS,2022,13(1):4994. |
| APA | Tian, Tian.,Wang, Yong-Jiang.,Huang, Jian-Ping.,Li, Jie.,Xu, Bingyan.,...&Huang, Sheng-Xiong.(2022).Catalytic innovation underlies independent recruitment of polyketide synthases in cocaine and hyoscyamine biosynthesis.NATURE COMMUNICATIONS,13(1),4994. |
| MLA | Tian, Tian,et al."Catalytic innovation underlies independent recruitment of polyketide synthases in cocaine and hyoscyamine biosynthesis".NATURE COMMUNICATIONS 13.1(2022):4994. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。