Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG
文献类型：期刊论文
| 作者 | Luo, Lan; Song, Xuemei; Chang, Xiao; Huang, Sheng; Zhou, Yunxi; Yang, Shengmei; Zhu, Yan; Zhang, Lanyan; Wu, Yongsheng; Zhang, Junyin |
| 刊名 | MOLECULES
 |
| 出版日期 | 2022 |
| 卷号 | 27期号:17页码:5691 |
| 关键词 | active ingredients polysaccharide purification glucan mannan immunomodulator BACILLUS-CALMETTE-GUERIN (BCG)-INDUCED MACROPHAGE CYTOTOXICITY IMMUNOMODULATORY ACTIVITY MALDI-TOF INTERLEUKIN-10 ACTIVATION IMMUNITY GLUCANS SULFATE MANNAN |
| DOI | 10.3390/molecules27175691 |
| 英文摘要 | Bacillus Calmette-Guerin polysaccharide and nucleic acid (BCG-PSN), extracted from Mycobacterium bovis, is an immunoregulatory medicine commonly used in clinic. However, the structural characteristics and potential pharmacological efficacy of the polysaccharides from BCG-PSN remain unclear. Herein, two polysaccharides (BCG-1 and BCG-2) were purified and their structures were characterized. Monosaccharide composition analysis combined with methylation analysis and NMR data indicated that BCG-1 and BCG-2 were an alpha-D-(1 -> 4)-mannan with (1 -> 2)-linked branches, and an alpha-D-(1 -> 4)-glucan with (1 -> 6)-linked branches, respectively. Herein, the mannan from BCG-PSN was first reported. Bioactivity assays showed that BCG-1 and BCG-2 dose-dependently and potently increased the production of inflammatory mediators (NO, TNF-alpha, IL-6, IL-1 beta, and IL-10), as well as their mRNA expressions in RAW264.7 cells; both have similar or stronger effects compared with BCG-PSN injection. These data suggest that BCG-1 and BCG-2 are very likely the active ingredients of BCG-PSN. |
| WOS记录号 | WOS:000851767500001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74673]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Luo, Lan,Song, Xuemei,Chang, Xiao,et al. Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG[J]. MOLECULES,2022,27(17):5691. |
| APA | Luo, Lan.,Song, Xuemei.,Chang, Xiao.,Huang, Sheng.,Zhou, Yunxi.,...&Wu, Mingyi.(2022).Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG.MOLECULES,27(17),5691. |
| MLA | Luo, Lan,et al."Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG".MOLECULES 27.17(2022):5691. |
入库方式： OAI收割
来源：昆明植物研究所
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