Engineering an oilseed crop for hyper-accumulation of carotenoids in the seeds without using a traditional marker gene
文献类型：期刊论文
| 作者 | He, Ming-Xia ; Wang, Jie-Lin; Lin, Yuan-Yuan; Huang, Jun-Chao; Liu, Ai-Zhong; Chen, Feng
|
| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2022 |
| 卷号 | 41期号:8页码:1751-1761 |
| 关键词 | Camelina sativa Carotenoid beta-Carotene Astaxanthin Marker gene 9-CIS-EPOXYCAROTENOID DIOXYGENASE GENE PHYTOENE SYNTHASE GENE TRANSGENIC PLANTS BETA-CAROTENE KETOCAROTENOID BIOSYNTHESIS CAMELINA-SATIVA ASTAXANTHIN CAULIFLOWER OVEREXPRESSION ARABIDOPSIS |
| DOI | 10.1007/s00299-022-02889-4 |
| 英文摘要 | Camelina sativa (L.) Crantz is an important oil crop with many excellent agronomic traits. This model oil plant has been exploited to accumulate value-added bioproducts using genetic manipulation that depends on antibiotic- or herbicide-based selection marker genes (SMG), one of the major concerns for genetically modified foods. Here we reported metabolic engineering of C. sativa to synthesize red ketocarotenoids that could serve as a reporter to visualize transgenic events without using a traditional SMG. Overexpression of a non-native beta-carotene ketolase gene coupled with three other carotenogenous genes (phytoene synthase, beta-carotene hydroxylase, and Orange) in C. sativa resulted in production of red seeds that were visibly distinguishable from the normal yellow ones. Constitutive expression of the transgenes led to delayed plant development and seed germination. In contrast, seed-specific transformants demonstrated normal growth and seed germination despite the accumulation of up to 70-fold the level of carotenoids in the seeds compared to the controls, including significant amounts of astaxanthin and keto-lutein. As a result, the transgenic seed oils exhibited much higher antioxidant activity. No significant changes were found in the profiles of fatty acids between transgenic and control seeds. This study provided an interesting tool for metabolic engineering of seed crops without using a disputed SMG. |
| WOS记录号 | WOS:000815412600001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74700]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | He, Ming-Xia,Wang, Jie-Lin,Lin, Yuan-Yuan,et al. Engineering an oilseed crop for hyper-accumulation of carotenoids in the seeds without using a traditional marker gene[J]. PLANT CELL REPORTS,2022,41(8):1751-1761. |
| APA | He, Ming-Xia,Wang, Jie-Lin,Lin, Yuan-Yuan,Huang, Jun-Chao,Liu, Ai-Zhong,&Chen, Feng.(2022).Engineering an oilseed crop for hyper-accumulation of carotenoids in the seeds without using a traditional marker gene.PLANT CELL REPORTS,41(8),1751-1761. |
| MLA | He, Ming-Xia,et al."Engineering an oilseed crop for hyper-accumulation of carotenoids in the seeds without using a traditional marker gene".PLANT CELL REPORTS 41.8(2022):1751-1761. |
入库方式： OAI收割
来源：昆明植物研究所
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