Comparative Metabolomic Profiling of the Metabolic Differences of Delta 9-Tetrahydrocannabinol and Cannabidiol
文献类型：期刊论文
| 作者 | Rao, Qianru; Zhang, Ting; Dai, Manyun; Li, Bin; Pu, Qianlun; Zhao, Min; Cheng, Yan; Yan, Dongmei; Zhao, Qi ; Wu, Zhanxuan E.
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| 刊名 | MOLECULES
 |
| 出版日期 | 2022 |
| 卷号 | 27期号:21页码:7573 |
| 关键词 | cannabis Delta 9-tetrahydrocannabinol cannabidiol metabolomics CANNABINOIDS |
| DOI | 10.3390/molecules27217573 |
| 英文摘要 | More than one hundred cannabinoids have been found in cannabis. Delta 9-Tetrahydrocannabinol (THC) is the recognized addictive constituent in cannabis; however, the mechanisms underlying THC-induced toxicity remain elusive. To better understand cannabis-induced toxicity, the present study compared the metabolic pathways of THC and its isomer cannabidiol (CBD) in human and mouse liver microsomes using the metabolomic approach. Thirty-two metabolites of THC were identified, including nine undescribed metabolites. Of note, two glutathione (GSH) and two cysteine (Cys) adducts were found in THC's metabolism. Molecular docking revealed that THC conjugates have a higher affinity with GSH and Cys than with the parent compound, THC. Human recombinant cytochrome P450 enzymes, and their corresponding chemical inhibitors, demonstrated that CYP3A4 and CYP1B1 were the primary enzymes responsible for the formation of THC-GSH and THC-Cys, thus enabling conjugation to occur. Collectively, this study systematically compared the metabolism of THC with the metabolism of CBD using the metabolomic approach, which thus highlights the critical role of metabolomics in identifying novel drug metabolites. Moreover, this study also facilitates mechanistic speculation in order to expand the knowledge of drug metabolism and safety. |
| WOS记录号 | WOS:000884041700001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74702]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Rao, Qianru,Zhang, Ting,Dai, Manyun,et al. Comparative Metabolomic Profiling of the Metabolic Differences of Delta 9-Tetrahydrocannabinol and Cannabidiol[J]. MOLECULES,2022,27(21):7573. |
| APA | Rao, Qianru.,Zhang, Ting.,Dai, Manyun.,Li, Bin.,Pu, Qianlun.,...&Li, Fei.(2022).Comparative Metabolomic Profiling of the Metabolic Differences of Delta 9-Tetrahydrocannabinol and Cannabidiol.MOLECULES,27(21),7573. |
| MLA | Rao, Qianru,et al."Comparative Metabolomic Profiling of the Metabolic Differences of Delta 9-Tetrahydrocannabinol and Cannabidiol".MOLECULES 27.21(2022):7573. |
入库方式： OAI收割
来源：昆明植物研究所
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