Epidemic Identification of Fungal Diseases in Morchella Cultivation across China
文献类型：期刊论文
| 作者 | Shi, Xiaofei; Liu, Dong; He, Xinhua; Liu, Wei; Yu, Fuqiang |
| 刊名 | JOURNAL OF FUNGI
 |
| 出版日期 | 2022 |
| 卷号 | 8期号:10页码:1107 |
| 关键词 | Clonostachys solani Diploospora longispora ITS amplicon sequencing fungal disease morels cultivation SPECIES COMPLEX |
| DOI | 10.3390/jof8101107 |
| 英文摘要 | True morels (Morchella, Pezizales) are world-renowned edible mushrooms (ascocarps) that are widely demanded in international markets. Morchella has been successfully artificially cultivated since 2012 in China and is rapidly becoming a new edible mushroom industry occupying up to 16,466 hectares in the 2021-2022 season. However, nearly 25% of the total cultivation area has annually suffered from fungal diseases. While a variety of morel pathogenic fungi have been reported their epidemic characteristics are unknown, particularly in regional or national scales. In this paper, ITS amplicon sequencing and microscopic examination were concurrently performed on the morel ascocarp lesions from 32 sites in 18 provinces across China. Results showed that Diploospora longispora (75.48%), Clonostachys solani (5.04%), Mortierella gamsii (0.83%), Mortierella amoeboidea (0.37%) and Penicillium kongii (0.15%) were the putative pathogenic fungi. The long, oval, septate conidia of D. longispora was observed on all ascocarps. Oval asexual spores and sporogenic structures, such as those of Clonostachys, were also detected in C. solani infected samples with high ITS read abundance. Seven isolates of D. longispora were isolated from seven selected ascocarps lesions. The microscopic characteristics of pure cultures of these isolates were consistent with the morphological characteristics of ascocarps lesions. Diploospora longispora had the highest amplification abundance in 93.75% of the samples, while C. solani had the highest amplification abundance in six biological samples (6.25%) of the remaining two sampling sites. The results demonstrate that D. longispora is a major culprit of morel fungal diseases. Other low-abundance non-host fungi appear to be saprophytic fungi infecting after D. longispora. This study provides data supporting the morphological and molecular identification and prevention of fungal diseases of morel ascocarps. |
| WOS记录号 | WOS:000873005000001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74705]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Shi, Xiaofei,Liu, Dong,He, Xinhua,et al. Epidemic Identification of Fungal Diseases in Morchella Cultivation across China[J]. JOURNAL OF FUNGI,2022,8(10):1107. |
| APA | Shi, Xiaofei,Liu, Dong,He, Xinhua,Liu, Wei,&Yu, Fuqiang.(2022).Epidemic Identification of Fungal Diseases in Morchella Cultivation across China.JOURNAL OF FUNGI,8(10),1107. |
| MLA | Shi, Xiaofei,et al."Epidemic Identification of Fungal Diseases in Morchella Cultivation across China".JOURNAL OF FUNGI 8.10(2022):1107. |
入库方式： OAI收割
来源：昆明植物研究所
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