Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa
文献类型：期刊论文
| 作者 | Liu, Yuanyuan; Zhang, Li; Li, Cheng; Yang, Yunqiang ; Duan, Yuanwen; Yang, Yongping; Sun, Xudong
|
| 刊名 | PLANT METHODS
 |
| 出版日期 | 2022 |
| 卷号 | 18期号:1页码:98 |
| 关键词 | Turnip Brassica rapa var rapa BrrWUSa Genetic transformation Genome editing EXPRESSION |
| DOI | 10.1186/s13007-022-00931-w |
| 英文摘要 | Background Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency. Results In this study, we established a transformation procedure involving chemical-inducible activation of the BrrWUSa gene, which resulted in high transformation frequencies of turnip. Estradiol-inducible BrrWUSa transgenic plants were fertile and showed no obvious developmental defects. Furthermore, we used CRISPR/Cas9 gene-editing technology to edit BrrTCP4b and generated 20 BrrTCP4b-edited seedlings with an increase in leaf trichome number. Conclusion The results demonstrate that BrrWUSa improves the regeneration efficiency in turnip. The transformation procedure represents a promising strategy to improve genetic transformation and for functional characterization of genes in turnip. |
| WOS记录号 | WOS:000836797200001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74787]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Yuanyuan,Zhang, Li,Li, Cheng,et al. Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa[J]. PLANT METHODS,2022,18(1):98. |
| APA | Liu, Yuanyuan.,Zhang, Li.,Li, Cheng.,Yang, Yunqiang.,Duan, Yuanwen.,...&Sun, Xudong.(2022).Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa.PLANT METHODS,18(1),98. |
| MLA | Liu, Yuanyuan,et al."Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa".PLANT METHODS 18.1(2022):98. |
入库方式： OAI收割
来源：昆明植物研究所
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