Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action
文献类型：期刊论文
| 作者 | Chen,Yuegui; Yan,Qin; Ji,Yunheng ; Bai,Xue; Li,Desen; Mu,Rongfang; Guo,Kai; Yang,Minjie; Tao,Yang; Gershenzon,Jonathan
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| 刊名 | ACTA PHARMACEUTICA SINICA B
 |
| 出版日期 | 2023 |
| 卷号 | 13期号:11页码:4638-4654 |
| 关键词 | Paris polyphylla var. yunnanensis Steroidal saponins Glycosyltransferases Sugar chain elongation Antifungal activity CHEMICAL-CONSTITUENTS GLYCOSYLTRANSFERASES GLYCOSIDES DIVERSITY CULTIVATION PATHWAY FAMILY PARTS GENUS STEP |
| ISSN号 | 2211-3843 |
| DOI | 10.1016/j.apsb.2023.05.033 |
| 英文摘要 | Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins. Here, we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris poly-phylla var. yunnanensis. Among them, a 20-O-rhamnosyltransferase and three 60-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides, respectively. These UDP-glycosyltransferases showed astonishing substrate promiscuity, resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds. A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular dock-ing simulations and protein model alignments, and a mutant UGT91AH1Y187A with increased catalytic efficiency was obtained. The steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes, and 20-O-rhamnosylation appeared to correlate with strong antifungal effects. The findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents. |
| WOS记录号 | WOS:001108208300001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74849]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Chen,Yuegui,Yan,Qin,Ji,Yunheng,et al. Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action[J]. ACTA PHARMACEUTICA SINICA B,2023,13(11):4638-4654. |
| APA | Chen,Yuegui.,Yan,Qin.,Ji,Yunheng.,Bai,Xue.,Li,Desen.,...&Li,Shenghong.(2023).Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action.ACTA PHARMACEUTICA SINICA B,13(11),4638-4654. |
| MLA | Chen,Yuegui,et al."Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action".ACTA PHARMACEUTICA SINICA B 13.11(2023):4638-4654. |
入库方式： OAI收割
来源：昆明植物研究所
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