Multiomics strategies for decoding seed dormancy breakdown in Paris polyphylla
文献类型：期刊论文
| 作者 | Zheng,Guowei; Li,Wenchun; Zhang,Shunzhen; Mi,Qi; Luo,Wenxiu; Zhao,Yanli; Qin,Xiangshi; Li,Weijiao; Pu,Shibiao; Xu,Furong |
| 刊名 | BMC PLANT BIOLOGY
 |
| 出版日期 | 2023 |
| 卷号 | 23期号:1页码:247 |
| 关键词 | Paris polyphylla Seed dormancy Metabolomics Transcriptomics Gibberellin A(3) Gibberellin A(4) ABSCISIC-ACID PROTEOMIC ANALYSIS GIBBERELLIN GERMINATION STRATIFICATION BIOSYNTHESIS ARABIDOPSIS REVEALS |
| DOI | 10.1186/s12870-023-04262-3 |
| 英文摘要 | BackgroundThe disruption of seed dormancy is a complicated process and is controlled by various factors. Among these factors, membrane lipids and plant hormones are two of the most important ones. Paris polyphylla is an important Chinese herbaceous species, and the dormancy trait of its seed limits the cultivation of this herb.ResultsIn this study, we investigate the global metabolic and transcriptomic profiles of Paris polyphylla during seed dormancy breaking. Widely targeted metabolomics revealed that lysophospholipids (lysoPLs) increased during P. polyphylla seed dormancy breaking. The expression of phospholipase A2 (PLA2), genes correlated to the production of lysoPLs, up-regulated significantly during this process. Abscisic acid (ABA) decreased dramatically during seed dormancy breaking of P. polyphylla. Changes of different GAs varied during P. polyphylla seeds dormancy breaking, 13-OH GAs, such as GA(53) were not detected, and GA(3) decreased significantly, whereas 13-H GAs, such as GA(15), GA(24) and GA(4) increased. The expression of CYP707As was not synchronous with the change of ABA content, and the expression of most UGTs, GA20ox and GA3ox up-regulated during seed dormancy breaking.ConclusionsThese results suggest that PLA2 mediated production of lysoPLs may correlate to the seed dormancy breaking of P. polyphylla. The conversion of ABA to ABA-GE catalysed by UGTs may be the main cause of ABA degradation. Through inhibition the expression of genes related to the synthesis of 13-OH GAs and up-regulation genes related to the synthesis of 13-H GAs, P. polyphylla synthesized more bioactive 13-H GA (GA(4)) to break its seed dormancy. |
| 学科主题 | Plant Sciences |
| WOS记录号 | WOS:000985362700003 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/74941]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Zheng,Guowei,Li,Wenchun,Zhang,Shunzhen,et al. Multiomics strategies for decoding seed dormancy breakdown in Paris polyphylla[J]. BMC PLANT BIOLOGY,2023,23(1):247. |
| APA | Zheng,Guowei.,Li,Wenchun.,Zhang,Shunzhen.,Mi,Qi.,Luo,Wenxiu.,...&Xu,Furong.(2023).Multiomics strategies for decoding seed dormancy breakdown in Paris polyphylla.BMC PLANT BIOLOGY,23(1),247. |
| MLA | Zheng,Guowei,et al."Multiomics strategies for decoding seed dormancy breakdown in Paris polyphylla".BMC PLANT BIOLOGY 23.1(2023):247. |
入库方式： OAI收割
来源：昆明植物研究所
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