A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts
文献类型：期刊论文
| 作者 | Xiahou,Zuo-Ying; Wambulwa,Moses C.; Xu,Zu-Chang; Ye,Lin-Jiang; Fan,Peng-Zhen; Magige,Ephie A.; Luo,Ya-Huang; Liu,Jie
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| 刊名 | PLANTS-BASEL
 |
| 出版日期 | 2023 |
| 卷号 | 12期号:24页码:4101 |
| 关键词 | genetic diversity Juglans microsatellite marker multiplex PCR transferability walnut CROSS-SPECIES AMPLIFICATION SSR-MARKERS JUGLANS-MANDSHURICA COMPUTER-PROGRAM DIVERSITY SOFTWARE SUCCESS ASSAY |
| ISSN号 | 2223-7747 |
| DOI | 10.3390/plants12244101 |
| 英文摘要 | Multiplex polymerase chain reaction (PCR) of microsatellite loci allows for simultaneous amplification of two or more pairs of primers in a single PCR reaction; hence, it is cost and time effective. However, very few attempts have been reported in non-model species. In this study, by combining a genome-based de novo development and cross-species application approach, a multiplex PCR system comprising 5 PCR reactions of 33 microsatellites consisting of 26 novel genomic and 7 literature-sourced loci was tested for polymorphisms, cross-species transferability, and the ability to assess genetic diversity and population structure of three walnut species (Juglans spp.). We found that the genome-based approach is more efficient than other methods. An allelic ladder was developed for each locus to enhance consistent genotyping among laboratories. The population genetic analysis results showed that all 33 loci were successfully transferred across the three species, showing high polymorphism and a strong genetic structure. Hence, the multiplex PCR system is highly applicable in walnut species. Furthermore, we propose an efficient pipeline to characterize and genotype polymorphic microsatellite loci. The novel toolbox developed here will aid future ecology and evolution studies in walnut and could serve as a model for other plant species. |
| 学科主题 | 199 |
| WOS记录号 | WOS:001136192300001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75055]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Xiahou,Zuo-Ying,Wambulwa,Moses C.,Xu,Zu-Chang,et al. A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts[J]. PLANTS-BASEL,2023,12(24):4101. |
| APA | Xiahou,Zuo-Ying.,Wambulwa,Moses C..,Xu,Zu-Chang.,Ye,Lin-Jiang.,Fan,Peng-Zhen.,...&Liu,Jie.(2023).A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts.PLANTS-BASEL,12(24),4101. |
| MLA | Xiahou,Zuo-Ying,et al."A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts".PLANTS-BASEL 12.24(2023):4101. |
入库方式： OAI收割
来源：昆明植物研究所
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