Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland
文献类型：期刊论文
| 作者 | Xu,Maonian; Liu,Yingkui; Moller,Erik; LaGreca,Scott; Moya,Patricia; Wang,Xinyu; Timdal,Einar; de Boer,Hugo; Barreno,Eva; Wang,Lisong
|
| 刊名 | MYCOKEYS
 |
| 出版日期 | 2023 |
| 期号 | 96页码:57-75 |
| 关键词 | Melanelia mtSSU Parmeliaceae PCR primer design INTERNAL TRANSCRIBED SPACER PCR PRIMERS REGION SEQUENCE |
| ISSN号 | 1314-4049 |
| DOI | 10.3897/mycokeys.96.100037 |
| 英文摘要 | The fungal mitochondrial small subunit (mtSSU) ribosomal DNA is one of the most commonly used loci for phylogenetic analysis of lichen-forming fungi, but their primer specificity to mycobionts has not been evaluated. The current study aimed to design mycobiont-specific mtSSU primers and highlights their utility with an example from the saxicolous lichen-forming fungal genus Melanelia Essl. in Iceland. The study found a 12.5% success rate (3 out of 24 specimens with good-quality mycobiont mtSSU sequences) using universal primers (i.e. mrSSU1 and mrSSU3R), not including off-target amplification of environ-mental fungi, e.g. Cladophialophora carrionii and Lichenothelia convexa. New mycobiont-specific primers (mt-SSU-581-5' and mt-SSU-1345-3') were designed by targeting mycobiont-specific nucleotide sites in comparison with environmental fungal sequences, and assessed for mycobiont primer specificity using in silico PCR. The new mycobiont-specific mtSSU primers had a success rate of 91.7% (22 out of 24 speci-mens with good-quality mycobiont mtSSU sequences) on the studied Melanelia specimens. Additional testing confirmed the specificity and yielded amplicons from 79 specimens of other Parmeliaceae myco-biont lineages. This study highlights the effectiveness of designing mycobiont-specific primers for studies on lichen identification, barcoding and phylogenetics. |
| 学科主题 | Mycology |
| WOS记录号 | WOS:000964240800001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75060]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Xu,Maonian,Liu,Yingkui,Moller,Erik,et al. Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland[J]. MYCOKEYS,2023(96):57-75. |
| APA | Xu,Maonian.,Liu,Yingkui.,Moller,Erik.,LaGreca,Scott.,Moya,Patricia.,...&Heidmarsson,Starri.(2023).Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland.MYCOKEYS(96),57-75. |
| MLA | Xu,Maonian,et al."Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland".MYCOKEYS .96(2023):57-75. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。