中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Advancing In-Depth N-Terminomics Detection with a Cleavable 2-Pyridinecarboxyaldehyde Probe

文献类型：期刊论文

作者Song, Xiaohan5; Ren, Xuelian4,5; Mei, Qi2,3; Liu, Hong1; Huang, He4,5
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版日期2024-02-29
卷号146期号:10页码:6487-6492
ISSN号0002-7863
DOI10.1021/jacs.4c02222
通讯作者Liu, Hong(hliu@simm.ac.cn) ; Huang, He(hhuang@simm.ac.cn)
英文摘要Proteolysis, an irreversible post-translational modification catalyzed by proteases, plays a crucial role in various biological processes. Exploring abnormally hydrolyzed proteins in pathological tissues is a valuable approach for elucidating the mechanisms underlying disease development. Herein, we have developed a cleavable 2-pyridinecarboxyaldehyde probe (2PCA-Probe) that enables efficient and in-depth N-terminomics detection, addressing limitations of previous methods. Furthermore, we unexpectedly discovered a new marker capable of identifying N-terminal chemical labeling with the 2PCA-Probe and elucidated the reaction mechanism. Using this probe, we identified 4686 N-terminal peptides in colorectal cancer and adjacent tissues, significantly expanding the depth of the N-terminome and revealing the potential role of abnormal protein hydrolysis in colorectal cancer development.
WOS关键词PROTEIN ; COMPLEX ; ROLES ; SITES
资助项目National Natural Science Foundation of China[22277125] ; National Natural Science Foundation of China[92253306] ; National Natural Science Foundation of China[23ZR1474600] ; Natural Science Foundation of Shanghai ; Shanghai Municipal Science and Technology Major Project
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001178614500001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/310124]  
专题新药研究国家重点实验室
通讯作者Liu, Hong; Huang, He
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
2.Shanxi Med Univ, Shanxi Bethune Hosp, Hosp 3, Canc Ctr, Taiyuan 030032, Peoples R China
3.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Oncol, Wuhan 430030, Peoples R China
4.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Song, Xiaohan,Ren, Xuelian,Mei, Qi,et al. Advancing In-Depth N-Terminomics Detection with a Cleavable 2-Pyridinecarboxyaldehyde Probe[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2024,146(10):6487-6492.
APA Song, Xiaohan,Ren, Xuelian,Mei, Qi,Liu, Hong,&Huang, He.(2024).Advancing In-Depth N-Terminomics Detection with a Cleavable 2-Pyridinecarboxyaldehyde Probe.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,146(10),6487-6492.
MLA Song, Xiaohan,et al."Advancing In-Depth N-Terminomics Detection with a Cleavable 2-Pyridinecarboxyaldehyde Probe".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 146.10(2024):6487-6492.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。